Human Dysbindin ELISA Kit | DTNBP1 elisa kit

Human Dysbindin ELISA Kit

Cat. Num. AAA763402

• Gene Names: Dtnbp1; sdy; Bloc1s8; AW048963; dysbindin; 5430437B18Rik
• Reactivity: Human
• Synonyms: Dysbindin; Human Dysbindin ELISA Kit; DTNBP1/Dysbindin/Hermansky-Pudlak syndrome 7 protein/HPS7 protein/Hermansky-Pudlak syndrome 7 protein/HPS7 protein/Dysbindin-1/BLOC-1 subunit 8/Dystrobrevin-binding protein 1/My031/BLOC1S8/DBND/HPS7; DTNBP1 elisa kit

• Reactivity: Human
• Sequence Length: 352

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 78-5000pg/ml
• Sensitivity: 46.9pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for DTNBP1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-DTNBP1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-DTNBP1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the DTNBP1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of DTNBP1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 15208633
• NCBI GeneID: 94245
• NCBI Accession #: NP_080048.2
• NCBI GenBank Nucleotide #: NM_025772.4
• Molecular Weight: 30,582 Da
• NCBI Official Full Name: dysbindin
• NCBI Official Synonym Full Names: dystrobrevin binding protein 1
• NCBI Official Symbol: Dtnbp1
• NCBI Official Synonym Symbols: sdy; Bloc1s8; AW048963; dysbindin; 5430437B18Rik
• NCBI Protein Information: dysbindin
• UniProt Protein Name: Dysbindin
• Protein Family: Dysbindin
• UniProt Gene Name: Dtnbp1
• UniProt Synonym Gene Names: Bloc1s8; Sdy; BLOC-1 subunit 8; HPS7 protein homolog
• UniProt Entry Name: DTBP1_MOUSE

Uniprot Description

DTNBP1: The BLOC-1 complex is required for normal biogenesis of lysosome-related organelles, such as platelet dense granules and melanosomes. Plays a role in intracellular vesicle trafficking. Plays a role in synaptic vesicle trafficking and in neurotransmitter release. May be required for normal dopamine homeostasis in the cerebral cortex, hippocampus, and hypothalamus. Plays a role in the regulation of cell surface exposure of DRD2. Contributes to the regulation of dopamine signaling. May play a role in actin cytoskeleton reorganization and neurite outgrowth. May modulate MAPK8 phosphorylation. Part of the biogenesis of lysosome-related organelles complex 1 (BLOC-1). The BLOC-1 complex is composed of BLOC1S1, BLOC1S2, BLOC1S3, DTNBP1, MUTED, PLDN, CNO/cappuccino and SNAPIN. Binds to DTNA and DTNB but may not be a physiological binding partner (PubMed:16980328). Interacts with RNF151 and CMYA5. Identified in a complex with the adapter-related protein complex 3 (AP-3). Interacts with TRIM32, AP3M1 and AP3B2. Identified in a complex with the biogenesis of lysosome-related organelles complex 2 (BLOC-2). Interacts with the DNA-dependent protein kinase complex DNA-PK. Detected in brain, in neurons and in neuropil. Detected in dentate gyrus and in pyramidal cells of hippocampus CA2 and CA3. Belongs to the dysbindin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Vesicle

Cellular Component: axon; cell junction; cytoplasm; cytoplasmic vesicle; dendritic spine; endoplasmic reticulum; endoplasmic reticulum membrane; endosome; growth cone; membrane; neuron projection; nucleus; plasma membrane; postsynaptic density; postsynaptic membrane; sarcolemma; sarcoplasm; synapse; synaptic vesicle membrane

Molecular Function: protein binding

Biological Process: actin cytoskeleton reorganization; anterograde axon cargo transport; anterograde synaptic vesicle transport; blood coagulation; muscle development; neurite development; neurite morphogenesis; organelle organization and biogenesis; platelet dense granule organization and biogenesis; positive regulation of neurotransmitter secretion; regulation of dopamine receptor signaling pathway; regulation of dopamine secretion; regulation of JNK activity; response to stimulus