IL33 recombinant protein

Recombinant Human IL33 Protein

Cat. Num. AAA8249288

• Gene Names: IL33; DVS27; IL1F11; NF-HEV; NFEHEV; C9orf26
• Reactivity: Human
• Applications: ELISA, Western Blot, SDS-Page
• Purity: Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
• Synonyms: IL33; Recombinant Human IL33 Protein; C9orf26; IL1F11; NFHEV; Interleukin-33; IL-33; Interleukin-1 family member 11; IL-1F11; Nuclear factor from high endothelial venules; NF-HEV; IL33 recombinant protein

• Host: E Coli
• Reactivity: Human
• Purity/Purification: Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 muM filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 7.5.
• Sequence: MGSSHHHHHH SSGLVPRGSH MSITGISPIT EYLASLSTYN DQSITFALED ESYEIYVEDL KKDEKKDKVL LSYYESQHPS NESGDGVDGK MLMVTLSPTK DFWLHANNKE HSVELHKCEK PLPDQAFFVL HNMHSNCVSF ECKTDPGVFI GVKDNHLALI KVDSSENLCT ENILFKLSET
• Sequence Length: 228

• Applicable Applications for IL33 recombinant protein: ELISA (EIA),Western Blot (WB), SDS-PAGE, Mass Spectrometry (MS)
• Endotoxin: Less than 0.1 ng/mug (1 IEU/mug) as determined by LAL test.
• Directions for Use: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 mug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Preparation and Storage: Lyophilized protein should be stored at -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at -20 degree C for 3 months.

Related Product Information for IL33 recombinant protein

Recombinant Human IL33 Protein is produced by our E Coli expression system and the target gene encoding Ser112-Thr270 is expressed.

NCBI and Uniprot Product Information

• NCBI GI #: 313851030
• NCBI GeneID: 90865
• NCBI Accession #: NP_001186569.1
• NCBI GenBank Nucleotide #: NM_001199640.1
• UniProt Accession #: O95760; Q2YEJ5; B2R8L1; B4DJ35; B4E1Q9; D3DRI5; E7EAX4
• Molecular Weight: 16,250 Da
• NCBI Official Full Name: interleukin-33 isoform b
• NCBI Official Synonym Full Names: interleukin 33
• NCBI Official Symbol: IL33
• NCBI Official Synonym Symbols: DVS27; IL1F11; NF-HEV; NFEHEV; C9orf26
• NCBI Protein Information: interleukin-33
• UniProt Protein Name: Interleukin-33
• Protein Family: Interleukin
• UniProt Gene Name: IL33
• UniProt Synonym Gene Names: C9orf26; IL1F11; NFHEV; IL-33; IL-1F11; NF-HEV
• UniProt Entry Name: IL33_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that binds to the IL1RL1/ST2 receptor. The encoded protein is involved in the maturation of Th2 cells and the activation of mast cells, basophils, eosinophils and natural killer cells. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]

Uniprot Description

IL33: Cytokine that binds to and signals through IL1RL1/ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Induces T-helper type 2-associated cytokines. Belongs to the IL-1 family. Highly divergent. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine

Chromosomal Location of Human Ortholog: 9p24.1

Cellular Component: extracellular region

Molecular Function: cytokine activity; protein binding

Biological Process: positive regulation of inflammatory response; positive regulation of macrophage activation; positive regulation of transcription from RNA polymerase II promoter; T-helper 2 type immune response