Western Blot (WB)
(The Cell lysates (40ug) and Mouse tissue extract (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Tubulin Beta 3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: 293T cell lysate Lane 3.: Mouse Brain tissue extract)
Mouse anti-Human Tubulin Beta 3 Monoclonal Antibody | anti-TUBB3 antibody
Tubulin Beta 3 antibody
Recommended dilution range for ICC/IF and Flow Cytometry is 1:200.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Western Blot (WB)
(The Cell lysates (40ug) and Mouse tissue extract (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Tubulin Beta 3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: 293T cell lysate Lane 3.: Mouse Brain tissue extract)
Western Blot (WB)
(The Cell lysates (40ug) and Mouse tissue extract (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Tubulin Beta 3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: 293T cell lysate Lane 3.: Mouse Brain tissue extract)
Immunofluorescence (IF)
(ICC/IF analysis of Tubulin Beta 3 in U-87MG cells, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human Tubulin Beta 3 antibody (1:200) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Immunofluorescence (IF)
(ICC/IF analysis of Tubulin Beta 3 in U-87MG cells, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human Tubulin Beta 3 antibody (1:200) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq, Oct 2010]
Uniprot Description
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and mantainance.
Research Articles on TUBB3
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Product Notes
The TUBB3 tubb3 (Catalog #AAA200355) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Tubulin Beta 3 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Tubulin Beta 3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS). WB: 1:1000. Recommended dilution range for ICC/IF and Flow Cytometry is 1:200. Researchers should empirically determine the suitability of the TUBB3 tubb3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Tubulin Beta 3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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