Interleukin 31 Receptor A Recombinant Protein | IL31RA recombinant protein

Interleukin 31 Receptor A, Recombinant, Human (IL-31RA, gp130-Like Monocyte Receptor, GLM-R, GPL)

Cat. Num. AAA650877

• Gene Names: IL31RA; CRL; GPL; CRL3; GLMR; GLM-R; PLCA2; hGLM-R; IL-31RA; PRO21384; MGC125346
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Highly Purified; 95%, as determined by SDS-PAGE and visualized by silver stain. Endotoxin 1EU.
• Synonyms: Interleukin 31 Receptor A; Recombinant; Human (IL-31RA; gp130-Like Monocyte Receptor; GLM-R; GPL); IL31RA recombinant protein

• Reactivity: Human
• Purity/Purification: Highly Purified; 95%, as determined by SDS-PAGE and visualized by silver stain. Endotoxin 1EU.
• Form/Format: Supplied as a lyophilized powder in PBS. Reconstitute with sterile PBS to 0.1mg/ml.

• Applicable Applications for IL31RA recombinant protein: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot and ELISA.; Dilution: Western Blot: 57.49kD protein migrates as an ~ 95-115kD protein in SDS-PAGE under reducing conditions.; ELISA: type binding assay with an estimated KD < nM.
• Preparation and Storage: Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile PBS and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for IL31RA recombinant protein

The interleukin-31 receptor A subunit (IL-31 RA), also known as gp130-Like Monocyte Receptor (GLM-R or GPL), is a ~100kD type I transmembrane glycoprotein that is classified as being a type I cytokine receptor (1, 2). A heterodimeric complex of IL-31 RA and the oncostatin M receptor (OSM-R) functions as the signaling receptor for IL-31 (3). Both subunits are inducibly expressed throughout the myelomonocytic lineage and are upregulated by interferon- and bacterial lipopolysaccharides (1-3). IL-31 RA is also expressed on keratinocytes, dorsal root ganglia neurons, and variably on lung epithelial cells (3-6). The 732 amino acid (aa) IL-31 RA contains a 19 aa signal sequence, a 500 aa extracellular domain (ECD), a 21 aa transmembrane domain and a 192 aa cytoplasmic domain. The ECD shares 60%, 58%, 73% and 70% aa identity with mouse, rat, dog and cow IL-31 RA ECD, respectively. Human IL-31 receptors do not respond to mouse IL-31 (7). The ECD contains five fibronectin type III domains; the first two contain four conserved cysteine residues and a WSXWS motif common to type I cytokine receptors (2). Twelve alternately spliced human IL-31 RA isoforms are known and range in size from 356-745 amino acids. A long (745 aa) and a short (560 aa) transmembrane form are the predominant forms, and many cell lines express both forms (8). The long form, like the 732 aa form, signals by recruiting STAT3, 5 or 1, while the short form does not recruit STATs and inhibits IL-31 signaling. The ratio of these forms and their coexpression with OSM-R determines a cell's response to IL-31 (8). In both humans and transgenic mice, IL-31 from skin-homing Th2 cells may contribute to the pruritis (itching) associated with nonatopic dermatitis, especially in infected skin (3, 9, 10).

Product Categories/Family for IL31RA recombinant protein

Growth Factors; Cytokines; Growth Factors-Interleukin

NCBI and Uniprot Product Information

• NCBI GI #: 119575329
• NCBI GeneID: 133396
• UniProt Accession #: Q8NI17; Q2TBA1; Q6EBC3; Q6EBC4; Q6EBC5; Q6EBC6; Q6UWL8; Q8WYJ0; A6NIF8
• Molecular Weight: 57.49kD
• NCBI Official Full Name: interleukin 31 receptor A, isoform CRA_e
• NCBI Official Synonym Full Names: interleukin 31 receptor A
• NCBI Official Symbol: IL31RA
• NCBI Official Synonym Symbols: CRL; GPL; CRL3; GLMR; GLM-R; PLCA2; hGLM-R; IL-31RA; PRO21384; MGC125346
• NCBI Protein Information: interleukin-31 receptor subunit alpha; zcytoR17; OTTHUMP00000122547; OTTHUMP00000195058; OTTHUMP00000195059; OTTHUMP00000195060; OTTHUMP00000221877; IL-31R subunit alpha; cytokine receptor-like 3; class I cytokine receptor; IL-31 receptor subunit alpha; gp130-like monocyte receptor; soluble type I cytokine receptor CRL3
• UniProt Protein Name: Interleukin-31 receptor subunit alpha
• Protein Family: Interleukin-31 receptor
• UniProt Gene Name: IL31RA
• UniProt Synonym Gene Names: CRL3; GPL
• UniProt Entry Name: IL31R_HUMAN

NCBI Description

The protein encoded by this gene belongs to the type I cytokine receptor family. This receptor, with homology to gp130, is expressed on monocytes, and is involved in IL-31 signaling via activation of STAT-3 and STAT-5. It functions either as a monomer, or as part of a receptor complex with oncostatin M receptor (OSMR). Several alternatively spliced transcript variants encoding different isoforms have been noted for this gene.

Uniprot Description

Function: Associates with OSMR to form the interleukin-31 receptor which activates STAT3 and to a lower extent STAT1 and STAT5. May function in skin immunity. Ref.1 Ref.2 Ref.8 Ref.9 Ref.10

Subunit structure: Heterodimer with OSMR. Interacts with JAK1 and STAT3. Ref.2 Ref.10

Subcellular location: Cell membrane; Single-pass type I membrane protein

Probable.

Tissue specificity: Expressed at low levels in testis, ovary, brain, prostate, placenta, thymus, bone marrow, trachea and skin. Detected in all of the myelomonocytic lineage. Expressed in CD14- and CD56-positive blood cells and by macrophages (at protein level). Ref.1 Ref.2 Ref.8 Ref.11

Induction: Up-regulated in lesional keratinocytes of patients with atopic dermatitis. Up-regulated by IFNG/IFN-gamma and bacterial lipopolysaccharides (LPS). Ref.1 Ref.2 Ref.8 Ref.11

Post-translational modification: N-glycosylated. Ref.8

Sequence similarities: Belongs to the type I cytokine receptor family. Type 2 subfamily.Contains 5 fibronectin type-III domains.

Sequence caution: The sequence AAS86444.1 differs from that shown. Reason: Erroneous initiation. The sequence AAS86445.1 differs from that shown. Reason: Erroneous initiation.