Mouse Interleukin 29 (IL-29) ELISA Kit | IL29 elisa kit

Mouse Interleukin 29, IL-29 ELISA Kit

Cat. Num. AAA945577

• Gene Names: IFNL1; IL29; IL-29
• Reactivity: Mouse
• Synonyms: Interleukin 29 (IL-29); Mouse Interleukin 29; IL-29 ELISA Kit; Mouse Interleukin 29 (IL-29) ELISA Kit; IFNL1; IL-29; interferon; lambda 1; interleukin 29; IL29 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse IL-29. No significant cross-reactivity or interference between mouse IL-29 and analogues was observed.
• Sequence Length: 200

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 12.5 pg/ml-800 pg/ml
• Sensitivity: < 3.1 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL29 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL-29 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-29 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-29 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-29 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 26024325
• NCBI GeneID: 282618
• NCBI Accession #: NP_742152.1
• NCBI GenBank Nucleotide #: NM_172140.1
• UniProt Accession #: Q8IU54; Q17R34; A0AV25
• Molecular Weight: 21,898 Da
• NCBI Official Full Name: interferon lambda-1
• NCBI Official Synonym Full Names: interferon, lambda 1
• NCBI Official Symbol: IFNL1
• NCBI Official Synonym Symbols: IL29; IL-29
• NCBI Protein Information: interferon lambda-1; IFN-lambda-1; interleukin-29; cytokine Zcyto21; interferon-lambda-1; interleukin 29 (interferon, lambda 1)
• UniProt Protein Name: Interferon lambda-1
• UniProt Gene Name: IFNL1
• UniProt Synonym Gene Names: IL29; ZCYTO21; IFN-lambda-1; IL-29
• UniProt Entry Name: IFNL1_HUMAN

NCBI Description

This gene encodes a cytokine distantly related to type I interferons and the IL-10 family. This gene, interleukin 28A (IL28A), and interleukin 28B (IL28B) are three closely related cytokine genes that form a cytokine gene cluster on a chromosomal region mapped to 19q13. Expression of the cytokines encoded by the three genes can be induced by viral infection. All three cytokines have been shown to interact with a heterodimeric class II cytokine receptor that consists of interleukin 10 receptor, beta (IL10RB) and interleukin 28 receptor, alpha (IL28RA). [provided by RefSeq, Jul 2008]

Uniprot Description

IL29: Cytokine with immunomodulatory activity. May play a role in antiviral immunity. Up-regulates MHC class I antigen expression. Ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IL28RA. The ligand/receptor complex seems to signal through the Jak-STAT pathway. Belongs to the IL-28/IL-29 family.

Protein type: Secreted, signal peptide; Cell cycle regulation; Secreted; Cytokine

Chromosomal Location of Human Ortholog: 19q13.13

Cellular Component: extracellular space; extracellular region; interleukin-28 receptor complex

Molecular Function: cytokine activity; interleukin-28 receptor binding; receptor binding

Biological Process: positive regulation of immune response; positive regulation of transcription, DNA-dependent; positive regulation of JAK-STAT cascade; JAK-STAT cascade; negative regulation of T cell differentiation; negative regulation of cell proliferation; positive regulation of interferon-gamma production; negative regulation of interleukin-13 production; negative regulation of T-helper 2 type immune response; positive regulation of tyrosine phosphorylation of STAT protein; negative regulation of interleukin-5 production; positive regulation of MHC class I biosynthetic process; negative regulation of transcription, DNA-dependent; negative regulation of memory T cell differentiation; defense response to virus