Rat Interleukin-24 ELISA Kit | Il24 elisa kit

Rat Interleukin-24 ELISA Kit

Cat. Num. AAA763584

• Gene Names: Il24; FISP; Mda7; St16; Mda-7
• Reactivity: Rat
• Synonyms: Interleukin-24; Rat Interleukin-24 ELISA Kit; Il24/Interleukin-24/il-24; Il24 elisa kit

• Reactivity: Rat
• Sequence Length: 220

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 15.6-1000pg/ml
• Sensitivity: 9.375pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Il24 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Rat Il24 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat Il24 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat Il24 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Rat Il24 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 157057186
• NCBI GeneID: 93672
• NCBI Accession #: NP_444325.2
• NCBI GenBank Nucleotide #: NM_053095.2
• Molecular Weight: 20,812 Da
• NCBI Official Full Name: interleukin-24
• NCBI Official Synonym Full Names: interleukin 24
• NCBI Official Symbol: Il24
• NCBI Official Synonym Symbols: FISP; Mda7; St16; Mda-7
• NCBI Protein Information: interleukin-24
• UniProt Protein Name: Interleukin-24
• Protein Family: Interleukin
• UniProt Gene Name: Il24
• UniProt Synonym Gene Names: Mda7; IL-24; MDA-7
• UniProt Entry Name: IL24_MOUSE

Uniprot Description

IL24: Has antiproliferative properties on melanoma cells and may contribute to terminal cell differentiation. Belongs to the IL-10 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis

Cellular Component: endoplasmic reticulum; extracellular region; extracellular space

Molecular Function: cytokine activity; protein binding

Biological Process: apoptosis; immune response; inflammatory response; negative regulation of apoptosis; negative regulation of caspase activity; negative regulation of cell migration; negative regulation of cell proliferation; positive regulation of apoptosis; positive regulation of caspase activity; positive regulation of cell proliferation; positive regulation of JAK-STAT cascade; positive regulation of tyrosine phosphorylation of Stat3 protein; serine phosphorylation of STAT3 protein