Human interleukin 23, alpha subunit p19 ELISA Kit | IL23A elisa kit

Human Interleukin-23 subunit alpha, IL-23A ELISA Kit

Cat. Num. AAA706156

• Gene Names: IL23A; P19; SGRF; IL-23; IL-23A; IL23P19
• Reactivity: Human
• Synonyms: interleukin 23; alpha subunit p19; Human Interleukin-23 subunit alpha; IL-23A ELISA Kit; Human Interleukin-23 subunit alpha (IL-23A) Elisa kit; IL-23; IL-23A; IL23P19; MGC79388; P19; SGRF; JKA3 induced upon T-cell activation; interleukin 23 p19 subunit; IL23A elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human IL-23A. No significant cross-reactivity or interference between human IL-23A and analogues was observed.
• Sequence Length: 189

• Samples: Serum, plasma, cell culture supernates, tissue homogenates and cell lysates
• Assay Type: Quantitative Sandwich
• Detection Range: 62.5 pg/ml-4000 pg/ml
• Sensitivity: <15.6 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL23A elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL-23A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-23A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-23A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-23A bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 7706702
• NCBI GeneID: 51561
• NCBI Accession #: NP_057668.1
• NCBI GenBank Nucleotide #: NM_016584.2
• UniProt Accession #: Q9NPF7; Q6NZ80; Q6NZ82; Q9H2A5
• Molecular Weight: 20,730 Da
• NCBI Official Full Name: interleukin-23 subunit alpha
• NCBI Official Synonym Full Names: interleukin 23, alpha subunit p19
• NCBI Official Symbol: IL23A
• NCBI Official Synonym Symbols: P19; SGRF; IL-23; IL-23A; IL23P19
• NCBI Protein Information: interleukin-23 subunit alpha; IL-23-A; IL-23p19; IL-23 subunit alpha; interleukin 23 p19 subunit; interleukin-23 subunit p19; JKA3 induced upon T-cell activation; interleukin-six, G-CSF related factor
• UniProt Protein Name: Interleukin-23 subunit alpha
• Protein Family: Interleukin
• UniProt Gene Name: IL23A
• UniProt Synonym Gene Names: SGRF; UNQ2498/PRO5798; IL-23 subunit alpha; IL-23-A; IL-23p19
• UniProt Entry Name: IL23A_HUMAN

NCBI Description

This gene encodes a subunit of the heterodimeric cytokine interleukin 23 (IL23). IL23 is composed of this protein and the p40 subunit of interleukin 12 (IL12B). The receptor of IL23 is formed by the beta 1 subunit of IL12 (IL12RB1) and an IL23 specific subunit, IL23R. Both IL23 and IL12 can activate the transcription activator STAT4, and stimulate the production of interferon-gamma (IFNG). In contrast to IL12, which acts mainly on naive CD4(+) T cells, IL23 preferentially acts on memory CD4(+) T cells. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Associates with IL12B to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.3 PublicationsManual assertion based on experiment in:Ref.1