IL-1RA Recombinant Protein | IL1RN recombinant protein

RECOMBINANT HUMAN INTERLEUKIN-1RA

Cat. Num. AAA232274

• Gene Names: IL1RN; DIRA; IRAP; IL1F3; IL1RA; MVCD4; IL-1RN; IL-1ra; IL-1ra3; ICIL-1RA
• Applications: ELISA, Functional Assay, Western Blot
• Purity: >98% by SDS PAGE
• Synonyms: IL-1RA; RECOMBINANT HUMAN INTERLEUKIN-1RA; IL1RN recombinant protein

• Purity/Purification: >98% by SDS PAGE
• Form/Format: Purified recombinant protein - lyophilised
• Concentration: Total protein concentration 0.1mg/ml after reconstitution. (varies by lot)
• Sequence Length: 159

• Applicable Applications for IL1RN recombinant protein: ELISA (EIA), Functional Assays (FN), Western Blot (WB)
• Application Notes: ELISA: Recombinant human Interleukin-1RA may be used as the standard for ELISA applications with either a purified human IL-1RA antibody or a biotinylated human IL-1RA antibody.; Western Blot: Recombinant huma Interleukin-1RA may be used as a positive control for Western Blotiing applications with either a purified human IL-1RA antibody or a biotinylated human IL-1RA antibody.; ELISA: Minimum Dilution: 0.2; Maximum Dilution: 0.4ng/well; Functional Assays: Minimum Dilution: 20; Maximum Dilution: 40ng/ml; Western Blotting: Minimum Dilution: 1.5; Maximum Dilution: 3.0ng/lane
• Reconstitution: Reconstitute with 0.2ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution. For extended storage, the addition of 0.1% bovine serum albumin (BSA) is recommended.; Preparation
• Buffer Solution: TRIS buffered saline
• Target Species: Human
• Preparation and Storage: Prior to reconstitution store at 4 degree C. Following reconstitution store at -20 degree C. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 3 months from date of reconstitution

Related Product Information for IL1RN recombinant protein

Interleukin-1 receptor antagonist (IL-1RA) is a naturally occurring protein which inhibits the activity of IL-1 alpha and IL-1 beta by competitively blocking binding to cell surface receptors for IL-1. In an assay of dose dependent inhibition of IL-1 stimulation of D10S cells, the ED50 was expected to be 20-40ng/ml.

NCBI and Uniprot Product Information

• NCBI GI #: 10835147
• NCBI GeneID: 3557
• NCBI Accession #: NP_000568.1
• NCBI GenBank Nucleotide #: NM_000577.4
• UniProt Accession #: P18510; Q14628; Q53SC2; Q7RTZ4; Q96GD6; Q9UPC0; A8K4G1
• Molecular Weight: Protein Molecular Weight: 17kD/153 amino acids
• NCBI Official Full Name: interleukin-1 receptor antagonist protein isoform 3
• NCBI Official Synonym Full Names: interleukin 1 receptor antagonist
• NCBI Official Symbol: IL1RN
• NCBI Official Synonym Symbols: DIRA; IRAP; IL1F3; IL1RA; MVCD4; IL-1RN; IL-1ra; IL-1ra3; ICIL-1RA
• NCBI Protein Information: interleukin-1 receptor antagonist protein; IL1 inhibitor; IL1RN (IL1F3); intracellular IL-1 receptor antagonist type II; intracellular interleukin-1 receptor antagonist (icIL-1ra); type II interleukin-1 receptor antagonist
• UniProt Protein Name: Interleukin-1 receptor antagonist protein
• Protein Family: Interleukin-1 receptor antagonist protein
• UniProt Gene Name: IL1RN
• UniProt Synonym Gene Names: IL1F3; IL1RA; IL-1RN; IL-1ra; IRAP
• UniProt Entry Name: IL1RA_HUMAN

NCBI Description

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses. This gene and five other closely related cytokine genes form a gene cluster spanning approximately 400 kb on chromosome 2. A polymorphism of this gene is reported to be associated with increased risk of osteoporotic fractures and gastric cancer. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

IL1RN: Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. Binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2; however, the physiological relevance of the latter association is unsure. The intracellular form of IL1RN is predominantly expressed in epithelial cells. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Cytokine; Vesicle; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 2q14.2

Cellular Component: extracellular space; cytoplasm; plasma membrane; intracellular

Molecular Function: interleukin-1 Type I receptor antagonist activity; interleukin-1 receptor binding; interleukin-1 Type II receptor antagonist activity; interleukin-1, Type II receptor binding; cytokine activity; interleukin-1, Type I receptor binding; interleukin-1 receptor antagonist activity

Biological Process: response to drug; positive regulation of JNK activity; negative regulation of heterotypic cell-cell adhesion; negative regulation of glutamate secretion; response to glucocorticoid stimulus; carboxylic acid metabolic process; response to lipopolysaccharide; female pregnancy; sensory perception of pain; fever; chronic inflammatory response to antigenic stimulus; memory; response to starvation; insulin secretion; acute-phase response; immune response; negative regulation of cytokine and chemokine mediated signaling pathway; response to activity; lipid metabolic process; negative regulation of membrane potential; negative regulation of cell migration; negative regulation of apoptosis

Disease: Microvascular Complications Of Diabetes, Susceptibility To, 4; Gastric Cancer, Hereditary Diffuse; Osteomyelitis, Sterile Multifocal, With Periostitis And Pustulosis