Rabbit anti-Human, Rat Mitochondrial Pyruvate dehydrogenase kinase 1 Polyclonal Antibody | anti-PDK1 antibody

Anti-Mitochondrial Pyruvate dehydrogenase kinase 1 antibody

Cat. Num. AAA176328

• Reactivity: Human, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen affinity purified.
• Synonyms: Mitochondrial Pyruvate dehydrogenase kinase 1; Polyclonal Antibody; Anti-Mitochondrial Pyruvate dehydrogenase kinase 1 antibody; [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1; mitochondrial; pyruvate dehydrogenase kinase; isozyme 1; [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1 antibody; mitochondrial antibody; HGNC:8809 antibody; Mitochondrial pyruvate dehydrogenase kinase isoenzyme 1 antibody; PDH kinase 1 antibody; Pdk1 antibody; PDK1_HUMAN antibody; Pyruvate dehydrogenase kinase isoform 1 antibody; Pyruvate dehydrogenase kinase; isoenzyme 1 antibody; anti-PDK1 antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: No cross reactivity with other proteins.
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Sequence Length: 436

• Applicable Applications for anti-PDK1 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: WB: Concentration: 0.1-0.5ug/ml; Tested Species: Hu, Rat; IHC-P: Concentration: 0.5-1ug/ml; Tested Species: Hu; Predicted Species: Rat; Antigen Retrieval: By Heat; ; Tested Species: In-house tested species with positive results.; Predicted Species: Species predicted to be fit for the product based on sequence similarties.; By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20 mins is required for the staining of formalin/paraffin sections.; Other applications have not been tested.; Optimal dilutions should be determined by end users.
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Mitochondrial Pyruvate dehydrogenase kinase 1 (408-421aa WKHYNTNHEADDWC), different from the related rat sequence by one amino acid, and from the related mouse sequence by two amino acids.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti-Mitochondrial Pyruvate dehydrogenase kinase 1 antibody, MBS176328, Western blottingLane 1: Rat Heart Tissue LysateLane 2: Rat Skeletal Muscle Tissue LysateLane 3: HELA Cell LysateLane 4: M231 Cell LysateLane 5: COLO320 Cell LysateLane 6: SW620 Cell Lysate )

Immunohistochemistry (IHC)

(Anti-Mitochondrial Pyruvate dehydrogenase kinase 1 antibody, MBS176328, IHC(P)IHC(P): Human Prostatic Cancer Tissue )

Related Product Information for anti-PDK1 antibody

Description: Rabbit IgG polyclonal antibody for[Pyruvate dehydrogenase[lipoamide]] kinase isozyme 1, mitochondrial(PDK1) detection. Tested with WB, IHC-P in Human, Rat.
Background: PDK1(Pyruvate Dehydrogenase Kinase Isoenzyme 1), is an enzyme that in humans is encoded by the PDK1 gene. To find human PDKs, Gudi et al.(1995) used oligonucleotide primers to screen a human liver cDNA library by PCR. They identified and reported the deduced amino acid sequences of PDK1, PDK2, and PDK3. The human PDK1 gene encodes a protein with a predicted molecular mass of 49,244 Da that shares 93% identity with that of the rat PDK1 gene. Northern blot analysis showed that the PDK1 message was expressed predominantly in the heart.

NCBI and Uniprot Product Information

• NCBI GI #: 3183117
• NCBI GeneID: 5163
• UniProt Accession #: Q15118; Q308M4; B2R6T1; B7Z937; D3DPD8; E9PD65
• NCBI Official Full Name: [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial
• NCBI Official Synonym Full Names: pyruvate dehydrogenase kinase, isozyme 1
• NCBI Official Symbol: PDK1
• NCBI Protein Information: [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial; [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial; PDH kinase 1; pyruvate dehydrogenase kinase, isoenzyme 1; mitochondrial pyruvate dehydrogenase, lipoamide, kinase isoenzyme 1
• UniProt Protein Name: [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial
• Protein Family: [Pyruvate dehydrogenase
• UniProt Gene Name: PDK1
• UniProt Synonym Gene Names: PDHK1; PDH kinase 1
• UniProt Entry Name: PDK1_HUMAN

NCBI Description

Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jun 2013]

Uniprot Description

PDHK1: an ubiquitously expressed, atypical protein kinase associated with the mitochondrial matrix. The PDHKs play crucial roles in switching metabolic flux from oxidative phosphorylation towards glycolysis. PDHK1 is detected in heart, pancreatic islets, and skeletal muscles. Contains a HATPase_c catalytic domain, found in several ATP-binding proteins including protein histidine kinases (PHKs), PHDKs, DNA gyrase B, topoisomerases, heat shock proteins, and DNA mismatch repair proteins. PDHK regulates glucose oxidation through inhibitory phosphorylation of the E1 alpha subunit of the mitochondrial pyruvate dehydrogenase complex (PDHC) at any one of 3 inhibitory serine residues. Inhibitory sites 1, 2, and 3 correspond to S293, S300, and S232 in human PDHA1, respectively. Four PDHK isoenzymes have been described, each with different site specificity: all four phosphorylate sites 1 and 2 but at different rates; for site 1 PDHK2 >PDHK4 >PDHK1 >PDHK3; for site 2, PDHK3> PDHK4 > PDHK2 > PDHK1. Only PDHK1 phosphorylates site 3. PDHKs are recruited to the PDHC by binding to a lipoyl group covalently attached to the inner lipoyl domain of the E2 component. PDHA1 deficiency is the most common enzyme defect in patients with primary lactic acidosis. PDHK1 is a direct HIF-1 target gene. Suppression of PDH by PDHK inhibits the conversion of pyruvate to acetyl-CoA, attenuates mitochondrial respiration, and may contribute to the increased lactate production observed in many tumors. The PDH pathway is repressed in a majority of non-small cell lung carcinomas. Inhibited by AZD7545, dichloroacetate (DCA) and radicicol. Radicicol inhibits kinase activity by binding directly to the ATP-binding pocket of PDHK, similar to HSP90 from the same ATPase/kinase superfamily.

Protein type: Mitochondrial; EC 2.7.11.2; Kinase, protein; Protein kinase, atypical; ATYPICAL group; PDHK family

Chromosomal Location of Human Ortholog: 2q31.1

Cellular Component: mitochondrion; mitochondrial matrix; mitochondrial pyruvate dehydrogenase complex

Molecular Function: protein binding; pyruvate dehydrogenase (acetyl-transferring) kinase activity; ATP binding; protein kinase activity

Biological Process: cell proliferation; cellular metabolic process; glucose metabolic process; regulation of acetyl-CoA biosynthetic process from pyruvate; pyruvate metabolic process; induction of apoptosis by oxidative stress; protein amino acid phosphorylation