Immunohistochemistry (IHC)
(Anti-MCAM / CD146 antibody IHC staining of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
Rabbit anti-Human MCAM / CD146 Polyclonal Antibody | anti-MCAM antibody
Rabbit Polyclonal to Human MCAM / CD146
Gene Names
MCAM; CD146; MUC18
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Immunoaffinity Purified
Synonyms
MCAM / CD146; Polyclonal Antibody; Rabbit Polyclonal to Human MCAM / CD146; Anti-MCAM / CD146 Antibody (Tyr641) IHC-plus; MCAM; CD146; CD146 antigen; Gicerin; Melanoma adhesion molecule; MUC18; p1h12; Human MCAM; anti-MCAM antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Immunoaffinity Purified
Form/Format
PBS, 0.09% sodium azide
Concentration
0.25 mg/ml (varies by lot)
Sequence Length
646
Applicable Applications for anti-MCAM antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
Application Notes
IHC-P (10 ug/ml), WB (1:1000)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
Target Species
Human
Immunogen Description
KLH conjugated synthetic peptide corresponding to amino acid residues surrounding Y641 of human MCAM.
Immunogen Type
Synthetic peptide
Immunogen
MCAM / CD146 antibody was raised against kLH conjugated synthetic peptide corresponding to amino acid residues surrounding Y641 of human MCAM.
Antigen Modification
Tyr641
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.
Immunohistochemistry (IHC)
(Anti-MCAM / CD146 antibody IHC staining of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
Immunohistochemistry (IHC)
(Anti-MCAM / CD146 antibody IHC staining of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
Western Blot (WB)
(Western blot analysis of anti-MCAM Antibody (Y641) in A375 cell line lysates (35 ug/lane). MCAM (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of anti-MCAM Antibody (Y641) in A375 cell line lysates (35 ug/lane). MCAM (arrow) was detected using the purified Pab.)
Related Product Information for anti-MCAM antibody
MCAM (also termed CD146 or MUC18) is a cell-adhesion molecule belonging to the immunoglobulin gene superfamily. It mediates cell-cell interaction through heterophilic Mel-CAM/ligand adhesion. MCAM has been shown to expressed widely in normal and neoplastic human tissues. MUC18 expression is increased during prostate cancer initiation (high grade PIN) and progression to carcinoma, and in metastatic cell lines and metastatic carcinoma.
Product Categories/Family for anti-MCAM antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
57,605 Da
NCBI Official Full Name
cell surface glycoprotein MUC18
NCBI Official Synonym Full Names
melanoma cell adhesion molecule
NCBI Official Symbol
MCAM
NCBI Official Synonym Symbols
CD146; MUC18
NCBI Protein Information
cell surface glycoprotein MUC18; Gicerin; melanoma adhesion molecule; cell surface glycoprotein P1H12; melanoma-associated antigen A32; melanoma-associated antigen MUC18; S-endo 1 endothelial-associated antigen
UniProt Protein Name
Cell surface glycoprotein MUC18
Protein Family
UniProt Gene Name
MCAM
UniProt Synonym Gene Names
MUC18
UniProt Entry Name
MUC18_HUMAN
Uniprot Description
MCAM: Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Cell adhesion
Chromosomal Location of Human Ortholog: 11q23.3
Cellular Component: extracellular space; focal adhesion; integral to membrane; plasma membrane; nucleus; external side of plasma membrane
Biological Process: anatomical structure morphogenesis; glomerular filtration; angiogenesis; cell adhesion; positive regulation of cell migration
Research Articles on MCAM
Similar Products
Product Notes
The MCAM mcam (Catalog #AAA242650) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Polyclonal to Human MCAM / CD146 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MCAM / CD146 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Western Blot (WB). IHC-P (10 ug/ml), WB (1:1000) Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti.. Researchers should empirically determine the suitability of the MCAM mcam for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MCAM / CD146, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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