IL1R1 / CD121a recombinant protein

Recombinant Rat IL1R1 / CD121a Protein (His tag)

Cat. Num. AAA2547495

• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: IL1R1 / CD121a; Recombinant Rat IL1R1 / CD121a Protein (His tag); Il1r1; IL1R1 / CD121a recombinant protein

• Host: Human Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 590

• Application Notes: The recombinant rat IL1R1 comprises 330 amino acids and predicts a molecular mass of 38.5 kDa. The apparent molecular mass of the rat IL1R1 is approximately 50-60 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Predicted N Terminal: Leu 34
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for IL1R1 / CD121a recombinant protein

Background: Interleukin 1 receptor, type I (IL-1R1) also known as CD121a (Cluster of Differentiation 121a), is an interleukin receptor. IL-1R1/CD121a is a cytokine receptor that belongs to the interleukin 1 receptor family. This protein is a receptor for interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I (IL1R1/IL1RA). IL-1R1/CD121a is an important mediator involved in many cytokine induced immune and inflammatory responses. This protein has been characterized by pharmacological and molecular techniques in the mouse brain. The spindle-shaped astrocytes enclose the wound, separating the healthy from damaged neural tissue. The shape change and subsequent repair processes are IL-1beta activity-dependent, acting through the IL-1 type 1 receptor (IL-1R1), as co-application of the IL-1type 1 receptor antagonist protein (IL-1ra) blocks IL-1beta induced effects. In the spleen, a slight increase in IL-1R AcP and IL-1R1 was observed during the first hours following LPS stimulation. In conclusion, IL-1R AcP mRNA is expressed in the brain and in other tissues where IL-1R1/CD121a transcripts are found. However, the regulation of its expression is distinct from IL-1R1/CD121a. The high level of expression and the lack of regulation of IL-1R AcP transcripts in the brain under inflammatory conditions suggest that the protein might be constitutively expressed in excess.

Description: A DNA sequence encoding the rat IL1R1 (NP_037255.3) extracellular domain (Met 1-Lys 352) was expressed, fused with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 148747433
• NCBI GeneID: 25663
• NCBI Accession #: NP_037255.3
• NCBI GenBank Nucleotide #: NM_013123.3
• Molecular Weight: 66,759 Da
• NCBI Official Full Name: interleukin-1 receptor type 1
• NCBI Official Synonym Full Names: interleukin 1 receptor type 1
• NCBI Official Symbol: Il1r1
• NCBI Protein Information: interleukin-1 receptor type 1
• UniProt Protein Name: Interleukin-1 receptor type 1
• UniProt Gene Name: Il1r1
• UniProt Synonym Gene Names: Il1ra; IL-1R-1; IL-1RT-1; IL-1RT1; IL-1R-alpha; mIL-1R1; mIL-1RI; sIL-1R1; sIL-1RI

NCBI Description

receptor for interleukin-1; involved in inflammatory response signalling [RGD, Feb 2006]

Uniprot Description

Receptor for IL1A, IL1B and IL1RN. After binding to interleukin-1 associates with the coreceptor IL1RAP to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B, MAPK and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Binds ligands with comparable affinity and binding of antagonist IL1RN prevents association with IL1RAP to form a signaling complex ().