IL1R1 / CD121a recombinant protein

Recombinant Human IL1R1 / CD121a Protein (His tag)

Cat. Num. AAA2546500

• Gene Names: IL1R1; P80; IL1R; IL1RA; CD121A; D2S1473; IL-1R-alpha
• Purity: > 97 % as determined by SDS-PAGE
• Synonyms: IL1R1 / CD121a; Recombinant Human IL1R1 / CD121a Protein (His tag); CD121A; D2S1473; IL-1R-alpha; IL1R; IL1RA; P80; IL1R1; IL1R1 / CD121a recombinant protein

• Host: Human Cells
• Purity/Purification: > 97 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 569

• Application Notes: The soluble form of recombinant human IL1R1 consists of 327 amino acids after removal of the signal peptide and has a predicted molecular mass of 37.7 kDa. In SDS-PAGE under reducing conditions, it migrates with an apparent molecular mass of 55-60 kDa due to glycosylation.
• Predicted N Terminal: Leu 18
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for IL1R1 / CD121a recombinant protein

Background: Interleukin 1 receptor, type I (IL-1R1) also known as CD121a (Cluster of Differentiation 121a), is an interleukin receptor. IL-1R1/CD121a is a cytokine receptor that belongs to the interleukin 1 receptor family. This protein is a receptor for interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I (IL1R1/IL1RA). IL-1R1/CD121a is an important mediator involved in many cytokine induced immune and inflammatory responses. This protein has been characterized by pharmacological and molecular techniques in the mouse brain. The spindle-shaped astrocytes enclose the wound, separating the healthy from damaged neural tissue. The shape change and subsequent repair processes are IL-1beta activity-dependent, acting through the IL-1 type 1 receptor (IL-1R1), as co-application of the IL-1type 1 receptor antagonist protein (IL-1ra) blocks IL-1beta induced effects. In the spleen, a slight increase in IL-1R AcP and IL-1R1 was observed during the first hours following LPS stimulation. In conclusion, IL-1R AcP mRNA is expressed in the brain and in other tissues where IL-1R1/CD121a transcripts are found. However, the regulation of its expression is distinct from IL-1R1/CD121a. The high level of expression and the lack of regulation of IL-1R AcP transcripts in the brain under inflammatory conditions suggest that the protein might be constitutively expressed in excess.

Description: A DNA sequence encoding the extracellular domain of human IL1R1 (NP_000868.1) (Met 1-Thr 332) was expressed with a fused polyhistidine tag at C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 4504659
• NCBI GeneID: 3554
• NCBI Accession #: NP_000868.1
• NCBI GenBank Nucleotide #: NM_000877.3
• UniProt Accession #: P14778; Q587I7
• Molecular Weight: 65,402 Da
• NCBI Official Full Name: interleukin-1 receptor type 1 isoform 1
• NCBI Official Synonym Full Names: interleukin 1 receptor type 1
• NCBI Official Symbol: IL1R1
• NCBI Official Synonym Symbols: P80; IL1R; IL1RA; CD121A; D2S1473; IL-1R-alpha
• NCBI Protein Information: interleukin-1 receptor type 1
• UniProt Protein Name: Interleukin-1 receptor type 1
• UniProt Gene Name: IL1R1
• UniProt Synonym Gene Names: IL1R; IL1RA; IL1RT1; IL-1R-1; IL-1RT-1; IL-1RT1; IL-1R-alpha; mIL-1R1; mIL-1RI; sIL-1R1; sIL-1RI

NCBI Description

This gene encodes a cytokine receptor that belongs to the interleukin-1 receptor family. The encoded protein is a receptor for interleukin-1 alpha, interleukin-1 beta, and interleukin-1 receptor antagonist. It is an important mediator involved in many cytokine-induced immune and inflammatory responses. This gene is located in a cluster of related cytokine receptor genes on chromosome 2q12. [provided by RefSeq, Dec 2013]

Uniprot Description

IL-1RA: Receptor for IL1A, IL1B and IL1RN. After binding to interleukin-1 associates with the corecptor IL1RAP to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B, MAPK and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Binds ligands with comparable affinity and binding of antagonist IL1RN prevents association with IL1RAP to form a signaling complex. Belongs to the interleukin-1 receptor family.

Protein type: EC 2.7.11.25; Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 2q11.2-q12.1

Cellular Component: integral to plasma membrane; membrane; plasma membrane

Molecular Function: interleukin-1 receptor activity; platelet-derived growth factor receptor binding; protein binding; signal transducer activity; transmembrane receptor activity

Biological Process: cell surface receptor linked signal transduction; immune response