IL1R1 / CD121a active protein

Recombinant Mouse IL1R1 / CD121a Protein (His tag)

Cat. Num. AAA2546780

• Gene Names: Il1r1; IL-iR; CD121a; CD121b; IL-1R1; Il1r-1
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: IL1R1 / CD121a; Recombinant Mouse IL1R1 / CD121a Protein (His tag); Interleukin-1 receptor type 1; IL-1RT1; IL-1 RI ; CD121b; IL-1R1; IL-iR; Il1r-1; IL1R1 / CD121a active protein

• Host: HEK293 Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4, 7.5% Trehalose
• Sequence: Met 1 - Lys 338

• Application Notes: The secreted recombinant mouse IL1R1 comprises 330 amino acids and has a calculated molecular mass of 38.7 kDa. As a result of glycosylation, the apparent molecular mass of the recombinant protein is approximately 55-60 kDa in SDS-PAGE under reducing conditions.
• Species: Mouse
• Tag: C-His
• Bio_activity: 1. Measured by its ability to bind mouse IL1B in a functional ELISA.2. Immobilized mouse IL1A at 10 ug/mL (100 ul/well) can bind mouse IL1R1. The EC50 of mouse IL1R1 is 0.13 ug/mL.
• Endotoxin: < 1.0 EU per ug of the protein as determined by the LAL method.
• Reconstitution: It is recommended that sterile water(400ul) be added to the vial to prepare a stock solution of 0.25mg/mL. Concentration is measured by UV-Vis.
• Preparation and Storage: Lyophilized proteins are stable for up to 12 months when stored at -20 to -80 degree C. Reconstituted protein solution can be stored at 4-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.; ; Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

SDS-PAGE

Related Product Information for IL1R1 / CD121a active protein

Interleukin 1 receptor, type I (IL-1R1) also known as CD121a (Cluster of Differentiation 121a), is an interleukin receptor. IL-1R1/CD121a is a cytokine receptor that belongs to the interleukin 1 receptor family. This protein is a receptor for interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I (IL1R1/IL1RA). IL-1R1/CD121a is an important mediator involved in many cytokine induced immune and inflammatory responses. This protein has been characterized by pharmacological and molecular techniques in the mouse brain. The spindle-shaped astrocytes enclose the wound, separating the healthy from damaged neural tissue. The shape change and subsequent repair processes are IL-1? activity-dependent, acting through the IL-1 type 1 receptor (IL-1R1), as co-application of the IL-1type 1 receptor antagonist protein (IL-1ra) blocks IL-1? induced effects. In the spleen, a slight increase in IL-1R AcP and IL-1R1 was observed during the first hours following LPS stimulation. In conclusion, IL-1R AcP mRNA is expressed in the brain and in other tissues where IL-1R1/CD121a transcripts are found. However, the regulation of its expression is distinct from IL-1R1/CD121a. The high level of expression and the lack of regulation of IL-1R AcP transcripts in the brain under inflammatory conditions suggest that the protein might be constitutively expressed in excess.

NCBI and Uniprot Product Information

• NCBI GI #: 6680417
• NCBI GeneID: 16177
• NCBI Accession #: NP_032388.1
• NCBI GenBank Nucleotide #: NM_008362.2
• UniProt Accession #: P13504
• Molecular Weight: Mol_Mass: 38.7 kDa; AP_Mol_Mass: 55-60 kDa
• NCBI Official Full Name: interleukin-1 receptor type 1 isoform 1
• NCBI Official Synonym Full Names: interleukin 1 receptor, type I
• NCBI Official Symbol: Il1r1
• NCBI Official Synonym Symbols: IL-iR; CD121a; CD121b; IL-1R1; Il1r-1
• NCBI Protein Information: interleukin-1 receptor type 1
• UniProt Protein Name: Interleukin-1 receptor type 1
• UniProt Gene Name: Il1r1
• UniProt Synonym Gene Names: Il-1r1; Il1ra; IL-1R-1; IL-1RT-1; IL-1RT1; IL-1R-alpha; mIL-1R1; mIL-1RI; sIL-1R1; sIL-1RI

Uniprot Description

IL-1RA: Receptor for IL1A, IL1B and IL1RN. After binding to interleukin-1 associates with the corecptor IL1RAP to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B, MAPK and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Binds ligands with comparable affinity and binding of antagonist IL1RN prevents association with IL1RAP to form a signaling complex. Belongs to the interleukin-1 receptor family.

Protein type: EC 2.7.11.25; Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 1 B|1 18.81 cM

Cellular Component: axon; cell surface; extracellular space; membrane; nucleus; postsynaptic density; protein complex

Molecular Function: interleukin-1 binding; interleukin-1 receptor activity; ionotropic glutamate receptor binding; platelet-derived growth factor receptor binding; protease binding; protein binding; signal transducer activity

Biological Process: cytokine and chemokine mediated signaling pathway; heat acclimation; regulation of inflammatory response; response to radiation