Interleukin 1 Beta (IL1b) Recombinant Protein | IL1b recombinant protein
Recombinant Interleukin 1 Beta (IL1b)
Gene Names
IL1B; IL1-BETA
Applications
SDS-Page, Western Blot, ELISA, Immunoprecipitation
Purity
> 95%
Synonyms
Interleukin 1 Beta (IL1b); Recombinant Interleukin 1 Beta (IL1b); IL1b recombinant protein
Host
E Coli
Purity/Purification
> 95%
Form/Format
Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Sequence
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHH HHSSGLVPRG SHMASMTGGQ QMGRGSEF-VRSLHCRLQD AQQKSLVLSG TYELKALHLN AENLNQQVVF SMSFVQGEES NDKIPVALGL RGKNLYLSCV MKDDKPTLQL ESVDPNRYPK KKMEKRFVFN KIEIKDKLEF ESAQFPNWYI STSQTEYMPV FLGNNSGGQD LIDFSMEFVS S
MGSSHHHH HHSSGLVPRG SHMASMTGGQ QMGRGSEF-VRSLHCRLQD AQQKSLVLSG TYELKALHLN AENLNQQVVF SMSFVQGEES NDKIPVALGL RGKNLYLSCV MKDDKPTLQL ESVDPNRYPK KKMEKRFVFN KIEIKDKLEF ESAQFPNWYI STSQTEYMPV FLGNNSGGQD LIDFSMEFVS S
Sequence Length
268
Applicable Applications for IL1b recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Organism
Oryctolagus cuniculus (Rabbit)
Expression System
Prokaryotic expression
Residues
Val118~Ser268 (Accession # P14628) with two N-terminal Tags, His-tag and T7-tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Reconstitution
Reconstitute in sterile PBS, pH7.2-pH7.4.
Preparation and Storage
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
SDS-Page
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
21.1kDa
NCBI Official Full Name
interleukin-1 beta
NCBI Official Symbol
IL1B
NCBI Official Synonym Symbols
IL1-BETA
NCBI Protein Information
interleukin-1 beta; IL-1 beta; interleukin 1 beta; lymphocyte proliferation-potentiating factor
UniProt Protein Name
Interleukin-1 beta
Protein Family
UniProt Gene Name
IL1B
UniProt Synonym Gene Names
IL-1 beta
UniProt Entry Name
IL1B_RABIT
Uniprot Description
Function: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
Subunit structure: Monomer.
Subcellular location: Secreted. Note: The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.
Domain: The similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function.
Sequence similarities: Belongs to the IL-1 family.
