Typical Testing Data/Standard Curve (for reference only)
Rat IL-18 ELISA Kit | IL18 elisa kit
Rat IL-18 PicoKine ELISA Kit
Gene Names
Il18; IL-18
Reactivity
Rat
Synonyms
IL-18; Rat IL-18 PicoKine ELISA Kit; Interleukin-18; interleukin 18; Iboctadekin; IFN gamma inducing factor; IFN-gamma-inducing factor; IGIF; IL 1 gamma; IL 18; IL 1g; IL-1 gamma; IL1 gamma; IL18; IL18 protein; IL18_HUMAN; IL1F4; IL1g; IL1gamma; ILIF4; Interferon gamma inducing factor; Interferon gamma-inducing factor; Interleukin 1 gamma; Interleukin 18; Interleukin-1 gamma; Interleukin18; MGC12320; IL18 elisa kit
Reactivity
Rat
Specificity
Natural and recombinant Rat IL-18
Sequence Length
194
Assay Type
Quantitative Sandwich
Detection Range
15.6pg/ml-1000pg/ml
Sensitivity
<1pg/ml
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for IL18 elisa kit
Principle of the Assay: Rat IL-18 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for IL-18 has been precoated onto 96-well plates. Standards(Expression system for standard: E.coli; Immunogen sequence: H37-S194) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-18 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin- Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat IL-18 amount of sample captured in plate.
Background: Interleukin(IL)-18, also called Interferon-gamma-inducing factor(IGIF), augments natural killer(NK) activity in spleen cells. The gene encodes a precursor protein of 192 amino acids and a mature protein of 157 amino acids.1 IL-18 is a recently discovered cytokine that modulates both T helper type 1(Th1) and Th2 responses.2 IL-18 is a potent proinflammatory cytokine with potential atherogenic properties. It is highly expressed in the atherosclerotic plaques compared with control normal arteries and is localized mainly in plaque macrophages.3
Background: Interleukin(IL)-18, also called Interferon-gamma-inducing factor(IGIF), augments natural killer(NK) activity in spleen cells. The gene encodes a precursor protein of 192 amino acids and a mature protein of 157 amino acids.1 IL-18 is a recently discovered cytokine that modulates both T helper type 1(Th1) and Th2 responses.2 IL-18 is a potent proinflammatory cytokine with potential atherogenic properties. It is highly expressed in the atherosclerotic plaques compared with control normal arteries and is localized mainly in plaque macrophages.3
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
20,056 Da
NCBI Official Full Name
Interleukin-18
NCBI Official Synonym Full Names
interleukin 18
NCBI Official Symbol
Il18
NCBI Official Synonym Symbols
IL-18
NCBI Protein Information
interleukin-18; IL-1 gamma; interleukin-1 gamma; IFN-gamma-inducing factor; interferon gamma-inducing factor; interferon-gamma-inducing factor
UniProt Protein Name
Interleukin-18
Protein Family
UniProt Gene Name
Il18
UniProt Synonym Gene Names
Igif; IL-18; IFN-gamma-inducing factor; IL-1 gamma
UniProt Entry Name
IL18_RAT
NCBI Description
a pro-inflammatory cytokine; acts as a modulator of immune functions [RGD, Feb 2006]
Uniprot Description
Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells.
Research Articles on IL18
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Product Notes
The Rat IL18 il18 (Catalog #AAA175814) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA175814 ELISA Kit recognizes Rat IL18. It is sometimes possible for the material contained within the vial of "IL-18, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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