Mouse Interleukin 18 (IL-18) ELISA Kit | IL-18 elisa kit

Mouse Interleukin 18 (IL-18) ELISA Kit

Cat. Num. AAA265871

• Gene Names: IL18; IGIF; IL-18; IL-1g; IL1F4
• Reactivity: Mouse
• Synonyms: Interleukin 18 (IL-18); Mouse Interleukin 18 (IL-18) ELISA Kit; IL-18 elisa kit

• Reactivity: Mouse
• Sequence Length: 193

• Samples: Mouse serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 1000 pg/mL-15.6 pg/mL
• Sensitivity: Up to 5 pg/mL
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for IL-18 elisa kit

Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Mouse IL-18 monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 4504653
• NCBI GeneID: 3606
• NCBI Accession #: NP_001553.1
• NCBI GenBank Nucleotide #: NM_001562.3
• Molecular Weight: 21,896 Da
• NCBI Official Full Name: interleukin-18 isoform 1 proprotein
• NCBI Official Synonym Full Names: interleukin 18
• NCBI Official Symbol: IL18
• NCBI Official Synonym Symbols: IGIF; IL-18; IL-1g; IL1F4
• NCBI Protein Information: interleukin-18; IL-1 gamma; iboctadekin; interleukin-1 gamma; IFN-gamma-inducing factor; interleukin 18 (interferon-gamma-inducing factor)
• UniProt Protein Name: Interleukin-18
• UniProt Gene Name: IL18
• UniProt Synonym Gene Names: IGIF; IL1F4; IL-18; IFN-gamma-inducing factor; IL-1 gamma
• UniProt Entry Name: IL18_HUMAN

NCBI Description

The protein encoded by this gene is a proinflammatory cytokine that augments natural killer cell activity in spleen cells, and stimulates interferon gamma production in T-helper type I cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2011]

Uniprot Description

IL18: Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells. Belongs to the IL-1 family.

Protein type: Cytokine

Chromosomal Location of Human Ortholog: 11q22.2-q22.3

Cellular Component: extracellular space; extracellular region; cytosol

Molecular Function: protein binding; cytokine activity

Biological Process: positive regulation of granulocyte macrophage colony-stimulating factor production; positive regulation of NK T cell proliferation; natural killer cell activation; positive regulation of NF-kappaB import into nucleus; positive regulation of activated T cell proliferation; chemokine biosynthetic process; cell-cell signaling; lipopolysaccharide-mediated signaling pathway; granulocyte macrophage colony-stimulating factor biosynthetic process; angiogenesis; inflammatory response; positive regulation of interleukin-17 production; regulation of cell adhesion; T-helper 1 type immune response; positive regulation of natural killer cell proliferation; interleukin-2 biosynthetic process; T-helper 2 type immune response; MAPKKK cascade; interleukin-13 biosynthetic process; sleep; positive regulation of tissue remodeling; positive regulation of interferon-gamma production; interferon-gamma biosynthetic process; immune response; negative regulation of myoblast differentiation; positive regulation of inflammatory response