Interleukin-6 ELISA Kit | IL-6 elisa kit

Interleukin-6 (IL-6)

Cat. Num. AAA590056

• Gene Names: IL6; HGF; HSF; BSF2; IL-6; IFNB2
• Synonyms: Interleukin-6; Interleukin-6 (IL-6); IL-6 elisa kit

• Sequence Length: 212

• Samples: Serum, Cell Culture Supernatant, and other biological fluids
• Assay Type: Sandwich
• Sensitivity: The minimum detectable dose of mouse IL-6 was determined by adding two standard deviations to the mean optical density value of 16 zero standard replicates and calculating the corresponding concentration from the standard curve. The minimum detectable dose of mouse IL-6 calculated from calibrate II diluted standard curve was <5.24pg/mL.
• Intra-assay Precision: To determine within-run precision, three different samples of known concentration were assayed by using 16 replicates in 1 assay.
• Inter-assay Precision: To determine between-run precision, three different samples of known concentration were assayed by using replicates on 8 different assays.
• Intended Uses: This Mouse IL-6 ELISA kit is to be used for the in vitro quantitative determination of mouse interleukin 6 (IL-6) concentrations in serum, cell culture supernatant, and other biological fluids. This kit is intended for LABORATORY RESEARCH USE ONLY.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL-6 elisa kit

Principle of the assay: This mouse IL-6 enzyme-linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for mouse IL-6. Standards or samples are then added to the appropriate microtiter plate wells and incubated. Mouse IL-6, if present, will bind and become immobilized by the antibody pre-coated on the wells. The microtiter plate wells are thoroughly washed to remove unbound mouse IL-6 and other components of the sample. In order to quantify the amount of mouse IL-6 present in the sample, a standardized preparation of biotin conjugated anti-mouse IL-6 detection antibody is added to each well. The detection antibody will bind to mouse IL-6 and be immobilized during the incubation. The plate is washed again and Avidin conjugated horseradish peroxidase (HRP) is added to each wells and incubated. Since Avidin has a very affinity to biotin, HRP will be linked indirectly to the detection antibody on the plate via the binding with of avidin to biotin. The microtiter plate is thoroughly washed to remove all unbound HRP and a TMB (3,3'5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain mouse IL-6 and will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. In order to measure the concentration of mouse IL-6 in the samples, this kit contains two calibration diluents (Calibrator Diluent I for serum/plasma testing and Calibrator Diluent II for cell culture supernatant/ urine testing). According to the testing system, the provided standard is diluted (2-fold) with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D.) versus IL-6 concentration (pg/mL). The concentration of IL-6 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Background: Interleukin 6 (IL-6) is a multifunctional cytokine secreted locally by a variety of cell types including T cells, B cells, monocytes/macrophages, endothelial cells, fibroblasts, hepatocytes, keratinocytes and astrocytes etc. IL-6 is up-regulated upon the binding of Toll-like receptors to antigen pattern ligands and by mitogenic stimulation during infection, acute phase reaction, trauma (especially burns) and malignancies. IL-6 secretion is significantly increased during physical excises. Also known as B-cell stimulatory factor-2 (BSF-2), IL-6 is an important cytokine for the differentiation of B-cells into immunoglobulin-secreting cells. In addition, IL-6 has been found to increase the number of platelets, influences cytotoxic T cell differentiation and activation, stimulate the differentiation and survival of neuronal cells, stimulate osteoclast formation, and induce terminal differentiation of M1 myeloid leukemic cells. IL-6 also stimulates the growth of hybridomas, plasmacytomas, myelomas, sarcomas, carcinomas, EBV-transformed B cells, keratinocytes, and mesangial cells. To melanoma cells and a few other cancer cells, IL-6 seemed to have an inhibitory effect. In acute inflammatory phase, IL-6 causes increased body temperature by initiating synthesis of Prostaglandin E2 in hypothalamus and IL-6 stimulates the synthesis of acute phase proteins. IL-6 plays an anti-inflammatory role in acute reaction by decreasing the secretion of TNF-alpha and IL-1. The anti-inflammatory activity is regulated via the classic signal pathway by the direct binding of IL-6 to its membrane receptor. In chronic inflammation, IL-6 plays a pro-inflammatory role. The transition is mediated by soluble IL- 6 receptor alpha (sIL-6Ralpha) via the Tran-signaling pathway. Human IL-6 and murine IL-6 exhibit approximately 65% sequence homology at the nucleotide level, and 42% homology at the amino acid level.

Product Categories/Family for IL-6 elisa kit

ELISA Kits for Mouse Cytokines and Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 10834984
• NCBI GeneID: 3569
• NCBI Accession #: NP_000591.1
• NCBI GenBank Nucleotide #: NM_000600.3
• Molecular Weight: 23,718 Da
• NCBI Official Full Name: interleukin-6
• NCBI Official Synonym Full Names: interleukin 6
• NCBI Official Symbol: IL6
• NCBI Official Synonym Symbols: HGF; HSF; BSF2; IL-6; IFNB2
• NCBI Protein Information: interleukin-6; B-cell differentiation factor; B-cell stimulatory factor 2; BSF-2; CDF; CTL differentiation factor; IFN-beta-2; hybridoma growth factor; interferon beta-2; interferon, beta 2; interleukin BSF-2
• UniProt Protein Name: Interleukin-6
• Protein Family: Interleukin
• UniProt Gene Name: IL6
• UniProt Entry Name: IL6_HUMAN

NCBI Description

This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. [provided by RefSeq, Jun 2011]

Uniprot Description

IL6: Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig- secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ). An inflammatory articular disorder with systemic- onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis. A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men. Belongs to the IL-6 superfamily.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 7p21

Cellular Component: extracellular space; cytoplasm; extracellular region; interleukin-6 receptor complex; external side of plasma membrane

Molecular Function: protein binding; interleukin-6 receptor binding; growth factor activity; cytokine activity

Biological Process: positive regulation of nitric oxide biosynthetic process; positive regulation of apoptosis; negative regulation of collagen biosynthetic process; positive regulation of transcription, DNA-dependent; negative regulation of cytokine secretion; response to glucocorticoid stimulus; positive regulation of JAK-STAT cascade; positive regulation of leukocyte chemotaxis; bone remodeling; glucose homeostasis; response to caffeine; activation of NF-kappaB transcription factor; positive regulation of tyrosine phosphorylation of Stat3 protein; response to antibiotic; muscle maintenance; monocyte chemotaxis; regulation of cell shape; positive regulation of T-helper 2 cell differentiation; positive regulation of acute inflammatory response; negative regulation of gluconeogenesis; acute-phase response; positive regulation of T cell proliferation; response to electrical stimulus; cell growth; defense response to virus; neurite development; response to drug; platelet activation; positive regulation of protein import into nucleus, translocation; positive regulation of interleukin-6 production; response to amino acid stimulus; defense response to protozoan; negative regulation of fat cell differentiation; positive regulation of chemokine production; defense response to Gram-positive bacterium; positive regulation of peptidyl-tyrosine phosphorylation; cell redox homeostasis; response to heat; positive regulation of B cell activation; neutrophil apoptosis; negative regulation of protein kinase activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; response to calcium ion; positive regulation of epithelial cell proliferation; negative regulation of apoptosis; negative regulation of muscle development; positive regulation of translation; positive regulation of smooth muscle cell proliferation; negative regulation of hormone secretion; neutrophil mediated immunity; regulation of circadian sleep/wake cycle, non-REM sleep; negative regulation of caspase activity; defense response to Gram-negative bacterium; response to insulin stimulus; negative regulation of cell proliferation; positive regulation of MAPKKK cascade; response to peptidoglycan; positive regulation of cell proliferation; hepatic immune response; inflammatory response; negative regulation of chemokine biosynthetic process; aging; response to nutrient levels; cytokine and chemokine mediated signaling pathway; positive regulation of immunoglobulin secretion; endocrine pancreas development; positive regulation of peptidyl-serine phosphorylation; regulation of angiogenesis; humoral immune response; positive regulation of osteoblast differentiation; positive regulation of protein kinase B signaling cascade; response to cold; positive regulation of neuron differentiation; positive regulation of DNA replication; positive regulation of transmission of nerve impulse

Disease: Rheumatoid Arthritis, Systemic Juvenile; Arteriovenous Malformations Of The Brain; Kaposi Sarcoma, Susceptibility To; Inflammatory Bowel Disease 1