Typical Testing Data/Standard Curve (for reference only)
Rat Interleukin-36 beta ELISA Kit | IL-36 beta/ IL-1F8/FIL-1h/IL-1H2 elisa kit
Rat Interleukin-36 beta ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: IL-36? ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-IL-36? antibody and an IL-36? -HRP conjugate. The assay sample and buffer are incubated together with IL-36?-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL-36? concentration since IL-36? from samples and IL-36? -HRP conjugate compete for the anti-IL-36? antibody binding site. Since the number of sites is limited, as more sites are occupied by IL-36? from the sample, fewer sites are left to bind IL-36?-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL-36? concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the interleukin 1 cytokine family. Protein structure modeling indicated that this cytokine may contain a 12-stranded beta-trefoil structure that is conserved between IL1A (IL-A alpha) and IL1B (IL-1 beta). This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. Two alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
Uniprot Description
IL36B: Stimulates production of interleukin-6 and interleukin-8 in synovial fibrobasts, articular chondrocytes and mature adipocytes. Induces expression of a number of antimicrobial peptides including beta-defensins 4 and 103 as well as a number of matrix metalloproteases. Belongs to the IL-1 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Cytokine
Chromosomal Location of Human Ortholog: 2q14
Cellular Component: extracellular space; extracellular region
Molecular Function: interleukin-1 receptor binding; cytokine activity
Biological Process: positive regulation of T cell differentiation; cytokine and chemokine mediated signaling pathway; innate immune response; positive regulation of interleukin-6 production; immune response; inflammatory response
Research Articles on IL-36 beta/ IL-1F8/FIL-1h/IL-1H2
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Product Notes
The Rat IL-36 beta/ IL-1F8/FIL-1h/IL-1H2 il36b (Catalog #AAA752661) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA752661 ELISA Kit recognizes Rat IL-36 beta/ IL-1F8/FIL-1h/IL-1H2. It is sometimes possible for the material contained within the vial of "Interleukin-36 beta, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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