Typical Testing Data/Standard Curve (for reference only)
Porcine Interleukin 31 ELISA Kit | IL-31 elisa kit
Porcine Interleukin 31 ELISA Kit
Gene Names
IL31; IL-31
Reactivity
Porcine
Synonyms
Interleukin 31; Porcine Interleukin 31 ELISA Kit; IL-31 elisa kit
Reactivity
Porcine
Specificity
This assay has high sensitivity and excellent specificity for detection of IL-31. No significant cross-reactivity or interference between IL-31 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IL-31 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
1.0 pg/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for IL-31 elisa kit
Intended Uses: This IL-31 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine IL-31. This ELISA kit for research use only!
Principle of the Assay: IL-31 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-IL-31 antibody and an IL-31-HRP conjugate. The assay sample and buffer are incubated together with IL-31-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL-31 concentration since IL-31 from samples and IL-31-HRP conjugate compete for the anti-IL-31 antibody binding site. Since the number of sites is limited, as more sites are occupied by IL-31 from the sample, fewer sites are left to bind IL-31-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL-31 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: IL-31 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-IL-31 antibody and an IL-31-HRP conjugate. The assay sample and buffer are incubated together with IL-31-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL-31 concentration since IL-31 from samples and IL-31-HRP conjugate compete for the anti-IL-31 antibody binding site. Since the number of sites is limited, as more sites are occupied by IL-31 from the sample, fewer sites are left to bind IL-31-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL-31 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for IL-31 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
18,205 Da
NCBI Official Full Name
interleukin-31
NCBI Official Synonym Full Names
interleukin 31
NCBI Official Symbol
IL31
NCBI Official Synonym Symbols
IL-31
NCBI Protein Information
interleukin-31
UniProt Protein Name
Interleukin-31
UniProt Gene Name
IL31
UniProt Synonym Gene Names
IL-31
UniProt Entry Name
IL31_HUMAN
NCBI Description
IL31, which is made principally by activated Th2-type T cells, interacts with a heterodimeric receptor consisting of IL31RA (MIM 609510) and OSMR (MIM 601743) that is constitutively expressed on epithelial cells and keratinocytes. IL31 may be involved in the promotion of allergic skin disorders and in regulating other allergic diseases, such as asthma (Dillon et al., 2004 [PubMed 15184896]).[supplied by OMIM, Mar 2008]
Uniprot Description
IL31: Activates STAT3 and possibly STAT1 and STAT5 through the IL31 heterodimeric receptor composed of IL31RA and OSMR. IL31 may function in skin immunity.
Protein type: Secreted; Secreted, signal peptide; Cytokine
Chromosomal Location of Human Ortholog: 12q24.31
Cellular Component: extracellular space
Molecular Function: cytokine activity
Biological Process: immune system process
Research Articles on IL-31
Similar Products
Product Notes
The Porcine IL-31 il31 (Catalog #AAA741997) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA741997 ELISA Kit recognizes Porcine IL-31. It is sometimes possible for the material contained within the vial of "Interleukin 31, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
