IGF2BP2 recombinant protein

IGF2BP2 Protein, Human, Recombinant (His & GST Tag)

Cat. Num. AAA8120690

• Gene Names: IGF2BP2; p62; IMP2; IMP-2; VICKZ2
• Purity: >76% as determined by SDS-PAGE
• Synonyms: IGF2BP2; IGF2BP2 Protein; Human; Recombinant (His & GST Tag); Human IGF2BP2/IMP2/p62 Protein (His & GST Tag); IMP-2 Protein; IMP2 Protein; VICKZ2 Protein; insulin-like growth factor 2 mRNA binding protein 2; IGF2BP2 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >76% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, 500mM NaCl, 0.5mM PMSF, 5mM Reduced Glutathione, pH8.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard co
• Sequence: Met1-Lys599

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Met
• Tag: N-GST & His
• Protein Construction: A DNA sequence encoding the human IGF2BP2 isoform 1 (Q9Y6M1-2)(Met 1-Lys 599) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for IGF2BP2 recombinant protein

Background: Insulin-like growth factor 2 mRNA-binding protein 2 (IGF2BP2) is a member of the IGF-II mRNA-binding protein (IMP) family. IGF2BP2 is a member of a family of mRNA binding proteins that, collectively, have been shown to bind to several different mRNAs in mammalian cells, including one of the mRNAs encoding insulin-like growth factor-2. Insulin-like growth factor 2 mRNA-binding protein 2 (IGF2BP2) is involved in the stimulation of insulin action. IGF2BP2/IMP2 is expressed in oocytes, granulosa cells of small and growing follicles, Leydig cells, spermatogonia, and semen (at protein level). It is also expressed in testicular cancer (at protein level). It is expressed weakly in the heart, placenta, skeletal muscle, bone marrow, colon, kidney, salivary glands, testis, and pancreas. IGF2BP2 binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. This binding is isoform-specific. IGF2BP2 may regulate the translation of target mRNAs.

Product Categories/Family for IGF2BP2 recombinant protein

G Protein-coupled Receptors Signaling

References

Omori S, et al. (2008) Association of CDKAL1, IGF2BP2, CDKN2A/B, HHEX, SLC30A8, and KCNJ11 with susceptibility to type 2 diabetes in a Japanese population. Diabetes. 57(3): 791-5.Grarup N, et al. (2007) Studies of association of variants near the HHEX, CDKN2A/B, and IGF2BP2 genes with type 2 diabetes and impaired insulin release in 10,705 Danish subjects: validation and extension of genome-wide association studies. Diabetes. 56(12): 3105-11.Wu Y, et al. (2008) Common variants in CDKAL1, CDKN2A/B, IGF2BP2, SLC30A8, and HHEX/IDE genes are associated with type 2 diabetes and impaired fasting glucose in a Chinese Han population. Diabetes. 57(10): 2834-42.

NCBI and Uniprot Product Information

• NCBI GI #: 56118219
• NCBI GeneID: 10644
• NCBI Accession #: NP_001007226.1
• NCBI GenBank Nucleotide #: NM_001007225.1
• UniProt Accession #: Q9Y6M1; A0A4Z0; B3FTN2; B3FTN3; B3FTN4
• Molecular Weight: 66,121 Da
• NCBI Official Full Name: insulin-like growth factor 2 mRNA-binding protein 2 isoform b
• NCBI Official Synonym Full Names: insulin-like growth factor 2 mRNA binding protein 2
• NCBI Official Symbol: IGF2BP2
• NCBI Official Synonym Symbols: p62; IMP2; IMP-2; VICKZ2
• NCBI Protein Information: insulin-like growth factor 2 mRNA-binding protein 2; VICKZ family member 2; IGF2 mRNA-binding protein 2; IGF-II mRNA-binding protein 2; hepatocellular carcinoma autoantigen p62
• UniProt Protein Name: Insulin-like growth factor 2 mRNA-binding protein 2
• Protein Family: Insulin-like growth factor 2 mRNA-binding protein
• UniProt Gene Name: IGF2BP2
• UniProt Synonym Gene Names: IMP2; VICKZ2; IGF2 mRNA-binding protein 2; IMP-2
• UniProt Entry Name: IF2B2_HUMAN

NCBI Description

This gene encodes a member of the IGF-II mRNA-binding protein (IMP) family. The protein encoded by this gene contains several four KH domains and two RRM domains. It functions by binding to the 5' UTR of the insulin-like growth factor 2 (IGF2) mRNA and regulating IGF2 translation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

IGF2BP2: Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binding is isoform-specific. May regulate translation of target mRNAs. Belongs to the RRM IMP/VICKZ family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; Translation

Chromosomal Location of Human Ortholog: 3q27.2

Cellular Component: cytoskeleton; cytoplasm; cytosol; nucleus

Molecular Function: protein binding; mRNA 3'-UTR binding; nucleotide binding; translation regulator activity; mRNA 5'-UTR binding

Biological Process: anatomical structure morphogenesis; mRNA transport; negative regulation of translation; gene expression; regulation of cytokine biosynthetic process

Disease: Diabetes Mellitus, Noninsulin-dependent