Typical Testing Data/Standard Curve (for reference only)
Human Insulin like Growth Factor 2, Antisense ELISA Kit | IGF2AS elisa kit
Human Insulin like Growth Factor 2, Antisense ELISA Kit
Gene Names
IGF2-AS; PEG8; IGF2AS; IGF2-AS1
Reactivity
Human
Synonyms
Insulin like Growth Factor 2; Antisense; Human Insulin like Growth Factor 2; Antisense ELISA Kit; IGF2AS elisa kit
Reactivity
Human
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
1.0-25ng/mL
Sensitivity
0.1ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for IGF2AS elisa kit
For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
INTENDED USE This IGF2AS ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human IGF2AS. This ELISA kit for research use only!
PRINCIPLE OF THE ASSAY IGF2AS ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IGF2AS antibody and an IGF2AS-HRP conjugate. The assay sample and buffer are incubated together with IGF2AS-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IGF2AS concentration since IGF2AS from samples and IGF2AS-HRP conjugate compete for the anti-IGF2AS antibody binding site. Since the number of sites is limited, as more sites are occupied by IGF2AS from the sample, fewer sites are left to bind IGF2AS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IGF2AS concentration in each sample is interpolated from this standard curve.
INTENDED USE This IGF2AS ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human IGF2AS. This ELISA kit for research use only!
PRINCIPLE OF THE ASSAY IGF2AS ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IGF2AS antibody and an IGF2AS-HRP conjugate. The assay sample and buffer are incubated together with IGF2AS-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IGF2AS concentration since IGF2AS from samples and IGF2AS-HRP conjugate compete for the anti-IGF2AS antibody binding site. Since the number of sites is limited, as more sites are occupied by IGF2AS from the sample, fewer sites are left to bind IGF2AS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IGF2AS concentration in each sample is interpolated from this standard curve.
Product Categories/Family for IGF2AS elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
18,035 Da
NCBI Official Full Name
IGF2AS protein
NCBI Official Synonym Full Names
IGF2 antisense RNA
NCBI Official Symbol
IGF2-AS
NCBI Official Synonym Symbols
PEG8; IGF2AS; IGF2-AS1
UniProt Protein Name
Putative insulin-like growth factor 2 antisense gene protein
UniProt Gene Name
IGF2-AS
UniProt Synonym Gene Names
IGF2-AS1; IGF2AS; IGF2-AS1
UniProt Entry Name
IG2AS_HUMAN
NCBI Description
This gene expresses a paternally imprinted antisense transcript of the insulin-like growth factor 2 gene. The transcript is overexpressed in Wilms' tumor. This gene is predicted to be non-coding because the predicted protein is not conserved in any other species and the majority of transcripts would be candidates for non-sense mediated decay (NMD) if a protein were expressed. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2009]
Uniprot Description
IGF2AS:
Chromosomal Location of Human Ortholog: 11p15.5
Research Articles on IGF2AS
Similar Products
Product Notes
The Human IGF2AS igf2-as (Catalog #AAA727138) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA727138 ELISA Kit recognizes Human IGF2AS. It is sometimes possible for the material contained within the vial of "Insulin like Growth Factor 2, Antisense, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
