Insulin-Like Growth Factor I Recombinant Protein | IGF1 recombinant protein

Recombinant Bovine Insulin-Like Growth Factor I

Cat. Num. AAA225400

• Gene Names: IGF1; IGF-1; IGF-I
• Reactivity: Bovine
• Applications: ELISA, Western Blot
• Purity: >95% by SDS PAGE analysis; Purified by ion-exchange chromatography
• Synonyms: Insulin-Like Growth Factor I; Recombinant Bovine Insulin-Like Growth Factor I; IGF-I; IGF1 recombinant protein

• Host: Pichia pastoris
• Reactivity: Bovine
• Purity/Purification: >95% by SDS PAGE analysis; Purified by ion-exchange chromatography
• Form/Format: Lyophilized in phosphate buffered saline with 10% Trahalose.
• Sequence Length: 154

• Applicable Applications for IGF1 recombinant protein: ELISA (EIA), Western Blot (WB)
• Reconstitution: Reconstitute with 20 ul sterile PBS containing 0.1% carrier protein. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. It is recommend that the vial is gently mixed after reconstitution.
• Preparation and Storage: Prior to reconstitution store at -20 degree C. After reconstitution store at -20 degree C.; Storage in frost-free freezers is not recommended. This product should be stored undiluted. Shelf Life: 12 months from date of despatch

ELISA (EIA)

(Bovine Insulin-Like Growth Factor I ELISA: Recombinant Bovine insulin-like growth factor I detected using Goat anti Bovine insulin-like growth factor I as the capture reagent and Goat anti Bovine insulin-like growth factor I:Biotin as the detection reagent followed by Streptavidin:HRP)

Related Product Information for IGF1 recombinant protein

Recombinant Bovine insulin-like growth factor I (IGF-I) is a recombinant protein corresponding to aa 50-119 of native bovine IGF-I.

IGF-I is a secreted mitogenic polypeptide and member of the insulin gene family, produced primarily by the liver, which effects a wide range of cells and plays a key role in cell proliferation and growth, inhibition of apoptosis, and is linked with tumor cell growth.

IGF-I signals by binding to the type 1 insulin-like growth factor receptor (IGF-IR) and also to the insulin receptor (IR), resulting in the activation of the AKT signaling pathway. IGF-I production is stimulated by growth hormone (GH) and can be hindered by GH insensitivity, deficiency in GH receptors or a malfunction in the post GH receptor signalling pathway.

NCBI and Uniprot Product Information

• NCBI GI #: 124247
• NCBI GeneID: 281239
• UniProt Accession #: P07455
• Molecular Weight: 7.6 kDa
• NCBI Official Full Name: Insulin-like growth factor I
• NCBI Official Synonym Full Names: insulin like growth factor 1
• NCBI Official Symbol: IGF1
• NCBI Official Synonym Symbols: IGF-1; IGF-I
• NCBI Protein Information: insulin-like growth factor I
• UniProt Protein Name: Insulin-like growth factor I
• Protein Family: Insulin-like growth factor
• UniProt Gene Name: IGF1
• UniProt Synonym Gene Names: IGF-I

Uniprot Description

The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation. Ca2+-dependent exocytosis of IGF1 is required for sensory perception of smell in the olfactory bulb. Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiatiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins ITGAV:ITGB3 and ITGA6:ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and IGFR1 are essential for IGF1 signaling. Induces the phosphorylation and activation of IGFR1, MAPK3/ERK1, MAPK1/ERK2 and AKT1 ().