IGF-I Recombinant Protein | Igf1 recombinant protein

RECOMBINANT MOUSE INSULIN-LIKE GROWTH FACTOR I

Cat. Num. AAA232345

• Gene Names: Igf1; Igf-1; Igf-I; C730016P09Rik
• Applications: ELISA, Functional Assay, Western Blot
• Purity: >97% by SDS PAGE and HPLC
• Synonyms: IGF-I; RECOMBINANT MOUSE INSULIN-LIKE GROWTH FACTOR I; Igf1 recombinant protein

• Purity/Purification: >97% by SDS PAGE and HPLC
• Form/Format: Purified recombinant protein - lyophilised
• Concentration: Approximate Protein Concentration: 1.0 mg/ml after reconstitution (varies by lot)
• Sequence Length: 153

• Applicable Applications for Igf1 recombinant protein: ELISA (EIA), Functional Assays (FN), Western Blot (WB)
• Application Notes: ELISA: Minimum Dilution: 0.2; Maximum Dilution: 0.4ng/well;; Functional Assays: Minimum Dilution: 0.1; Maximum Dilution: 1.0ng/ml;; Western Blotting: Minimum Dilution: 1.5; Maximum Dilution: 3.0ng/lane
• Reconstitution: Reconstitute with 10 ul distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. N.B. For functional studies do not add sodium azide.
• Target Species: Mouse
• Preparation and Storage: Prior to reconstitution store at 4 degree C. Following reconstitution store at -20 degree C. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 3 months from date of reconstitution.

Related Product Information for Igf1 recombinant protein

MBS232345 is an E Coli-derived recombinant protein corresponding to full-length mature murine insulin-like growth factor 1 (IGF-1), a 70 amino acid peptide that is structurally and functionally related to insulin but with higher growth-promoting activities. Recombinant mouse IGF-1 has an ED50 of

NCBI and Uniprot Product Information

• NCBI GI #: 163659894
• NCBI GeneID: 16000
• NCBI Accession #: NP_001104745.1
• NCBI GenBank Nucleotide #: NM_001111275.1
• UniProt Accession #: P05017; P05018; Q6LDP4; Q8C4U6
• Molecular Weight: Protein Molecular Weight: 7.6kD (70 amino acid sequence)
• NCBI Official Full Name: insulin-like growth factor I isoform 4 preproprotein
• NCBI Official Synonym Full Names: insulin-like growth factor 1
• NCBI Official Symbol: Igf1
• NCBI Official Synonym Symbols: Igf-1; Igf-I; C730016P09Rik
• NCBI Protein Information: insulin-like growth factor I; somatomedin
• UniProt Protein Name: Insulin-like growth factor I
• Protein Family: Insulin-like growth factor
• UniProt Gene Name: Igf1
• UniProt Synonym Gene Names: Igf-1; IGF-I
• UniProt Entry Name: IGF1_MOUSE

Uniprot Description

IGF1: The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency). IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation. Belongs to the insulin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted

Cellular Component: extracellular space; cell soma; extracellular region; intracellular

Molecular Function: integrin binding; insulin-like growth factor receptor binding; protein serine/threonine kinase activator activity; protein binding; growth factor activity; hormone activity; steroid binding; insulin receptor binding

Biological Process: positive regulation of transcription, DNA-dependent; regulation of protein metabolic process; chondroitin sulfate proteoglycan biosynthetic process; exocrine pancreas development; water homeostasis; positive regulation of glucose import; positive regulation of fibroblast proliferation; proteoglycan biosynthetic process; inner ear development; positive regulation of DNA binding; muscle hypertrophy; nervous system development; positive regulation of protein import into nucleus, translocation; positive regulation of mitosis; regulation of establishment and/or maintenance of cell polarity; regulation of calcium ion transport; positive regulation of cell growth; positive regulation of phosphoinositide 3-kinase cascade; cell activation; positive regulation of peptidyl-tyrosine phosphorylation; branching morphogenesis of a tube; insulin-like growth factor receptor signaling pathway; response to heat; regulation of gene expression; positive regulation of fat cell differentiation; positive regulation of transcription from RNA polymerase II promoter; alveolus development; positive regulation of epithelial cell proliferation; negative regulation of apoptosis; positive regulation of insulin-like growth factor receptor signaling pathway; myoblast proliferation; positive regulation of smooth muscle cell proliferation; positive regulation of glycogen biosynthetic process; negative regulation of caspase activity; detection of mechanical stimulus involved in sensory perception; positive regulation of smooth muscle cell migration; positive regulation of activated T cell proliferation; regulation of translation; negative regulation of cell proliferation; glial cell differentiation; positive regulation of MAPKKK cascade; mammary gland development; positive regulation of cell proliferation; regulation of steroid hormone receptor signaling pathway; positive regulation of granule cell precursor proliferation; regulation of protein amino acid phosphorylation; phosphoinositide-mediated signaling; multicellular organism growth; myotube cell development; satellite cell compartment self-renewal involved in skeletal muscle regeneration; memory; regulation of cell proliferation; myoblast differentiation; cell proliferation; positive regulation of protein kinase B signaling cascade; positive regulation of osteoblast differentiation; positive regulation of tyrosine phosphorylation of Stat5 protein; positive regulation of glycolysis; blood vessel remodeling; positive regulation of Ras protein signal transduction; cell development; positive regulation of DNA replication; lung development