Insulin Like Growth Factor-1 Des Active Protein | IGF1 active protein

Recombinant Human Insulin Like Growth Factor-1 Des (1-3)

Cat. Num. AAA143178

• Gene Names: IGF1; IGFI; IGF-I; IGF1A
• Purity: Greater than 97.0% as determined by: ; (a) Analysis by RP-HPLC.; (b) Analysis by SDS-PAGE.
• Synonyms: Insulin Like Growth Factor-1 Des; Recombinant Human Insulin Like Growth Factor-1 Des (1-3); IGF1 Human Des1-3; Insulin-Like Growth Factor 1 Des (1-3) Human Recombinant; Somatomedin C; IGF-I; IGFI; IGF1; IGF-IA; Mechano growth factor; MGF; Des(1-3); Des1-3; Des 1-3; Des (1-3); IGF-1 (4-70); IGF1 Des1-3; IGF1 active protein

• Purity/Purification: Greater than 97.0% as determined by: ; (a) Analysis by RP-HPLC.; (b) Analysis by SDS-PAGE.
• Form/Format: Sterile Filtered White lyophilized (freeze-dried) powder.; ; The protein (1mg/ml) was lyophilized was lyophilized from a 0.2µm filtered concentrated solution in 20mM PBS, pH 7.0.
• Sequence: TLCGAELVDA LQFVCGDRGF YFNKPTGYGS SSRRAPQTGI VDECCFRSCD LRRLEMYCAP LKPAKSA
• Sequence Length: 153

• Source: Escherichia Coli
• Solubility: It is recommended to reconstitute the IGF-I des(1-3) in sterile 18M-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
• Biological Activity: The ED₅₀as determined by a cell proliferation assay using FDC-P1 cells is less than 2.0 ng/ml, corresponding to a specific activity of > 5×10⁵units/mg.
• Preparation and Storage: Lyophilized IGF-I des(1-3) although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IGF1 des-1-3 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).; ; Please prevent freeze-thaw cycles.

Related Product Information for IGF1 active protein

GF-I Des(1-3) Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 67 amino acids (aa 4-70) and having a molecular mass of 7368.5 Dalton.

IGF-1 Des1-3 is purified by proprietary chromatographic techniques.

Product Categories/Family for IGF1 active protein

Cytokines and Growth Factors; Growth Factors; Insulin-Like Growth Factor

NCBI and Uniprot Product Information

• NCBI GI #: 11024682
• NCBI GeneID: 3479
• NCBI Accession #: NP_000609.1
• NCBI GenBank Nucleotide #: NM_000618.3
• UniProt Accession #: P01343; Q14620; B2RWM7; E9PD02; P05019
• NCBI Official Full Name: insulin-like growth factor I isoform 4 preproprotein
• NCBI Official Synonym Full Names: insulin-like growth factor 1 (somatomedin C)
• NCBI Official Symbol: IGF1
• NCBI Official Synonym Symbols: IGFI; IGF-I; IGF1A
• NCBI Protein Information: insulin-like growth factor I; IGF-IA; IGF-IB; MGF; insulin-like growth factor IA; insulin-like growth factor IB; mechano growth factor; somatomedin-C
• UniProt Protein Name: Insulin-like growth factor I
• Protein Family: Insulin-like growth factor
• UniProt Gene Name: IGF1
• UniProt Synonym Gene Names: IBP1; IGF-I; MGF
• UniProt Entry Name: IGF1_HUMAN

NCBI Description

The protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in this gene are a cause of insulin-like growth factor I deficiency. Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2009]

Uniprot Description

IGF1: The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency). IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation. Belongs to the insulin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 12q23.2

Cellular Component: insulin-like growth factor binding protein complex; extracellular space; plasma membrane; extracellular region

Molecular Function: integrin binding; insulin-like growth factor receptor binding; protein binding; growth factor activity; hormone activity; insulin receptor binding

Biological Process: muscle development; positive regulation of transcription, DNA-dependent; chondroitin sulfate proteoglycan biosynthetic process; glycolate metabolic process; exocrine pancreas development; water homeostasis; positive regulation of glucose import; positive regulation of fibroblast proliferation; proteoglycan biosynthetic process; inner ear development; positive regulation of DNA binding; muscle hypertrophy; platelet activation; positive regulation of mitosis; positive regulation of protein import into nucleus, translocation; regulation of establishment and/or maintenance of cell polarity; positive regulation of phosphoinositide 3-kinase cascade; cell activation; positive regulation of peptidyl-tyrosine phosphorylation; branching morphogenesis of a tube; insulin-like growth factor receptor signaling pathway; response to heat; regulation of gene expression; positive regulation of transcription from RNA polymerase II promoter; alveolus development; positive regulation of epithelial cell proliferation; negative regulation of apoptosis; positive regulation of insulin-like growth factor receptor signaling pathway; positive regulation of smooth muscle cell proliferation; myoblast proliferation; positive regulation of glycogen biosynthetic process; positive regulation of activated T cell proliferation; signal transduction; positive regulation of smooth muscle cell migration; negative regulation of cell proliferation; glial cell differentiation; platelet degranulation; positive regulation of MAPKKK cascade; mammary gland development; positive regulation of cell proliferation; DNA replication; skeletal development; positive regulation of granule cell precursor proliferation; phosphoinositide-mediated signaling; multicellular organism growth; myotube cell development; regulation of multicellular organism growth; satellite cell compartment self-renewal involved in skeletal muscle regeneration; myoblast differentiation; positive regulation of protein kinase B signaling cascade; cell proliferation; positive regulation of osteoblast differentiation; cellular protein metabolic process; positive regulation of tyrosine phosphorylation of Stat5 protein; Ras protein signal transduction; positive regulation of glycolysis; blood vessel remodeling; positive regulation of Ras protein signal transduction; blood coagulation; cell motility; positive regulation of DNA replication

Disease: Insulin-like Growth Factor I Deficiency