Rabbit E4BP4 Polyclonal Antibody | anti-E4BP4 antibody

E4BP4 (E65) Polyclonal Antibody

Cat. Num. AAA3002156

• Gene Names: NFIL3; E4BP4; IL3BP1; NFIL3A; NF-IL3A
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: E4BP4; Polyclonal Antibody; E4BP4 (E65) Polyclonal Antibody; E4 promoter binding protein 4; E4 promoter-binding protein 4; E4BP 4; E4BP4 protein; IL3BP 1; IL3BP1; Interleukin 3 binding protein 1; Interleukin 3 promoter transcriptional activator; Interleukin-3 promoter transcriptional activator; Interleukin-3-binding protein 1; NFIL 3; NFIL 3A; NFIL3; NFIL3A; Nuclear factor interleukin 3 regulated; Nuclear factor interleukin-3-regulated protein; Transcriptional activator NF IL3A; Transcriptional activator NF-IL3A; anti-E4BP4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: E4BP4 (E65) polyclonal antibody detects endogenous levels of E4BP4protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 462

• Applicable Applications for anti-E4BP4 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 32-86 of Human E4BP4.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of E4BP4 (E65) pAb at 1:500 dilutionLane1:A549 whole cell lysate (40ug) Lane2:SKOVCAR3 whole cell lysate (40ug) Lane3:A2780 whole cell lysate (40ug) Lane4:H9C2 whole cell lysate (40ug) Lane5:BV2 whole cell lysate (40ug))

Related Product Information for anti-E4BP4 antibody

E4BP4, also known as NFIL3, functions as a transcriptional repressor and is a member of the basic leucine zipper (bZIP) transcription factor family. E4BP4 binds with high specificity to the E4 ATF, which is a DNA sequence traditionally targeted by the ATF/CREB family of transcription factors. A 65 amino acid segment located in the carboxy-terminus of E4BP4 interacts specifically with the TBP binding protein Dr1. In the suprachiasmatic nucleus, circadian center and liver, E4BP4 competes with PAR proteins for DNA binding via a reciprocating mechanism. The phase expression of E4BP4 correlates with the circadian cycle and represses transcription of genes otherwise activated by PAR transcription regulators. E4BP4 also plays an important role in an IL-3-mediated signaling pathway that is responsible for the survival of B cell progenitors. The gene encoding human E4BP4 maps to chromosome 9q22.

NCBI and Uniprot Product Information

• NCBI GI #: 52630429
• NCBI GeneID: 4783
• NCBI Accession #: NP_005375.2
• NCBI GenBank Nucleotide #: NP_005375.2
• UniProt Accession #: Q16649
• Molecular Weight: ~ 60kDa
• NCBI Official Full Name: nuclear factor interleukin-3-regulated protein
• NCBI Official Synonym Full Names: nuclear factor, interleukin 3 regulated
• NCBI Official Symbol: NFIL3
• NCBI Official Synonym Symbols: E4BP4; IL3BP1; NFIL3A; NF-IL3A
• NCBI Protein Information: nuclear factor interleukin-3-regulated protein
• UniProt Protein Name: Nuclear factor interleukin-3-regulated protein
• UniProt Gene Name: NFIL3
• UniProt Synonym Gene Names: E4BP4; IL3BP1
• UniProt Entry Name: NFIL3_HUMAN

NCBI Description

The protein encoded by this gene is a transcriptional regulator that binds as a homodimer to activating transcription factor (ATF) sites in many cellular and viral promoters. The encoded protein represses PER1 and PER2 expression and therefore plays a role in the regulation of circadian rhythm. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Feb 2014]

Uniprot Description

E4BP4: Acts as a transcriptional regulator that recognizes and binds to the sequence 5'-[GA]TTA[CT]GTAA[CT]-3', a sequence present in many cellular and viral promoters. Represses transcription from promoters with activating transcription factor (ATF) sites. Represses promoter activity in osteoblasts. Represses transcriptional activity of PER1. Represses transcriptional activity of PER2 via the B- site on the promoter. Activates transcription from the interleukin-3 promoter in T-cells. Competes for the same consensus-binding site with PAR DNA-binding factors (DBP, HLF and TEF). Component of the circadian clock that acts as a negative regulator for the circadian expression of PER2 oscillation in the cell-autonomous core clock. Protects pro-B cells from programmed cell death. Belongs to the bZIP family. NFIL3 subfamily.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 9q22

Cellular Component: nucleus

Molecular Function: DNA binding; transcription corepressor activity; transcription factor activity

Biological Process: circadian rhythm; transcription from RNA polymerase II promoter; immune response; negative regulation of transcription from RNA polymerase II promoter