Goat Insulin-like growth factor-2 ELISA Kit | IGF-2 elisa kit

Goat Insulin-like growth factor-2 ELISA Kit

Cat. Num. AAA738473

• Gene Names: IGF2; IGF-II; PP9974; C11orf43
• Reactivity: Goat
• Synonyms: Insulin-like growth factor-2; Goat Insulin-like growth factor-2 ELISA Kit; IGF-2 elisa kit

• Reactivity: Goat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IGF-2 elisa kit

For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

INTENDED USE This IGF-2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat IGF-2. This ELISA kit for research use only!

PRINCIPLE OF THE ASSAY IGF-2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IGF-2 antibody and an IGF-2-HRP conjugate. The assay sample and buffer are incubated together with IGF-2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IGF-2 concentration since IGF-2 from samples and IGF-2-HRP conjugate compete for the anti-IGF-2 antibody binding site. Since the number of sites is limited, as more sites are occupied by IGF-2 from the sample, fewer sites are left to bind IGF-2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IGF-2 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for IGF-2 elisa kit

Cancer

NCBI and Uniprot Product Information

• NCBI GI #: 189955
• NCBI GeneID: 3481
• Molecular Weight: 26,331 Da
• NCBI Official Full Name: insulin-like growth factor II
• NCBI Official Synonym Full Names: insulin-like growth factor 2
• NCBI Official Symbol: IGF2
• NCBI Official Synonym Symbols: IGF-II; PP9974; C11orf43
• NCBI Protein Information: insulin-like growth factor II; insulin-like growth factor type 2; insulin-like growth factor 2 (somatomedin A)
• UniProt Protein Name: Insulin-like growth factor II
• UniProt Gene Name: IGF2
• UniProt Synonym Gene Names: PP1446; IGF-II
• UniProt Entry Name: IGF2_HUMAN

NCBI Description

This gene encodes a member of the insulin family of polypeptide growth factors, which are involved in development and growth. It is an imprinted gene, expressed only from the paternal allele, and epigenetic changes at this locus are associated with Wilms tumour, Beckwith-Wiedemann syndrome, rhabdomyosarcoma, and Silver-Russell syndrome. A read-through INS-IGF2 gene exists, whose 5' region overlaps the INS gene and the 3' region overlaps this gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2010]

Uniprot Description

IGF2: The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development. Epigenetic changes of DNA hypomethylation in IGF2 are a cause of Silver-Russell syndrome (SRS). A clinically heterogeneous condition characterized by severe intrauterine growth retardation, poor postnatal growth, craniofacial features such as a triangular shaped face and a broad forehead, body asymmetry, and a variety of minor malformations. The phenotypic expression changes during childhood and adolescence, with the facial features and asymmetry usually becoming more subtle with age. Belongs to the insulin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: extracellular space; extracellular region; plasma membrane

Molecular Function: insulin-like growth factor receptor binding; protein serine/threonine kinase activator activity; protein binding; growth factor activity; hormone activity; insulin receptor binding; receptor activator activity

Biological Process: response to nicotine; positive regulation of catalytic activity; genetic imprinting; multicellular organismal development; positive regulation of glycogen biosynthetic process; exocrine pancreas development; female pregnancy; positive regulation of activated T cell proliferation; response to estradiol stimulus; response to radiation; platelet degranulation; regulation of transcription, DNA-dependent; positive regulation of MAPKKK cascade; positive regulation of cell proliferation; skeletal development; response to drug; response to nutrient levels; platelet activation; positive regulation of mitosis; glucose metabolic process; memory; osteoblast differentiation; positive regulation of protein kinase B signaling cascade; positive regulation of peptidyl-tyrosine phosphorylation; organ morphogenesis; cellular protein metabolic process; response to ethanol; insulin receptor signaling pathway; striated muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of insulin receptor signaling pathway; positive regulation of protein amino acid phosphorylation; blood coagulation

Disease: Silver-russell Syndrome; Growth Restriction, Severe, With Distinctive Facies; Beckwith-wiedemann Syndrome; Wilms Tumor 1