IL28B Recombinant Protein | IFNL3 recombinant protein

IL28B Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8118873

• Gene Names: Ifnl3; Il28; IFL-1; Il28b; IL-28B; INF-alpha; INF-lambda
• Purity: >95 % as determined by SDS-PAGE
• Synonyms: IL28B; IL28B Protein; Mouse; Recombinant (His Tag); Mouse IL-28B/IFN-lambda-3 Protein (His Tag); interferon; lambda 3; IFL-1 Protein; IL-28B Protein; Il28 Protein; Il28b Protein; INF-alpha Protein; INF-lambda Protein; IFNL3 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Sequence Positions: 185aa
• Sequence: Met1-Val193

• Species: Mouse
• Tag: C-His
• Endotoxin: <1.0EU per ug protein as determined by the LAL method
• Predicted N Terminal: Asp20
• Protein Construction: |A DNA sequence encoding the mouse Ifnl3 (NP_796370.1) (Met1-Val193) was expressed with a polyhistidine tag at the C-terminus
• Activity: Measured in antiviral assay using HepG2 cells infected with vesicular stomatitis virus. The ED50 for this effect is typically 44.6-178.4ng/mL.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; Shipping: They are shipped out with blue ice pack

Testing Data

Testing Data

(Measured in antiviral assay using HepG2 cells infected with vesicular stomatitis virus. The ED50 for this effect is typically 44.6-178.4 ng/mL.)

Related Product Information for IFNL3 recombinant protein

Interleukin-28B (IL-28B) also known as Interferon lambda-3 and IFN-lambda-3, belongs to the type III interferon family of cytokines and are highly similar to IL-29. IL-28B belongs to the newly described interferon lambda (IFNlambda) family of cytokines. IL-28B is a cytokine with immunomodulatory activity. It functions in Up-regulating MHC class I antigen expression. IL-28B displays potent antiviral activity and antitumor activity. This cytokine serves as a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IL28RA. The ligand/receptor complex seems to signal through the Jak-STAT pathway. IL-28B, like IL-12, is capable of robustly enhancing adaptive immunity. Moreover, we describe for the first time how IL-28B reduces regulatory T-cell populations during DNA vaccination, whereas IL-12 increases this cellular subset. We also show that IL-28B, unlike IL-12, can increase the percentage of splenic CD8+ T cells in vaccinated animals and that these cells are more granular and have higher antigen-specific cytolytic degranulation compared with cells taken from animals that received IL-12 as an adjuvant.

References

Ge D, et al.. (2009) Genetic variation in IL28B predicts hepatitis C treatment-induced viral clearance. Nature. 461(7262): 399-401.Morrow MP, et al.. (2009) Comparative ability of IL-12 and IL-28B to regulate Treg populations and enhance adaptive cellular immunity. Blood. 113(23): 5868-77. Sheppard P, et al.. (2003) IL-28, IL-29 and their class II cytokine receptor IL-28R. Nat Immunol. 4(1): 63-8.

NCBI and Uniprot Product Information

• NCBI GI #: 28893579
• NCBI GeneID: 338374
• NCBI Accession #: NP_796370.1
• NCBI GenBank Nucleotide #: NM_177396.1
• UniProt Accession #: Q8CGK6; Q4VK73
• Molecular Weight: 21,664 Da
• NCBI Official Full Name: interferon lambda-3
• NCBI Official Synonym Full Names: interferon lambda 3
• NCBI Official Symbol: Ifnl3
• NCBI Official Synonym Symbols: Il28; IFL-1; Il28b; IL-28B; INF-alpha; INF-lambda
• NCBI Protein Information: interferon lambda-3; IFN-lambda-3; interferon alpha; interleukin 28; interleukin 28B; interleukin-28B
• UniProt Protein Name: Interferon lambda-3
• Protein Family: Interferon
• UniProt Gene Name: Ifnl3
• UniProt Synonym Gene Names: Il28; Il28b; IFN-lambda-3; IL-28B
• UniProt Entry Name: IFNL3_MOUSE

Uniprot Description

IL-28B: Cytokine with immunomodulatory activity. Up-regulates MHC class I antigen expression. Displays potent antiviral activity. Also displays antitumor activity. Ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IL28RA. The ligand/receptor complex seems to signal through the Jak-STAT pathway. Belongs to the IL-28/IL-29 family.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: extracellular space; extracellular region

Molecular Function: cytokine activity; receptor binding

Biological Process: positive regulation of immune response; innate immune response; JAK-STAT cascade; defense response to virus