IFN-gamma recombinant protein

Mouse IFN-gamma protein, His Tag

Cat. Num. AAA9718980

• Gene Names: Ifng; Ifg; IFN-g
• Purity: >95 % as determined by SDS-PAGE
• Synonyms: IFN-gamma; Mouse IFN-gamma protein; His Tag; IFG; IFI; IFN gamma; Interferon Gamma; Interferon gamma; IFNG; IFN-gamma recombinant protein

• Host: E Coli
• Purity/Purification: >95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 150mM NaCl, 20mM Tris, pH8.0.
• Sequence: His23-Cys155

• Protein Length Note: The recombinant Mouse IFN-gamma protein consists of 154 amino acids and predicts a molecular mass of 16.9 kDa.
• Amino Acid Note: Amino acid sequence derived from Mouse IFN-gamma protein soluble form (NP_P01580) (His23-Cys155) was expressed, was expressed with a polyhistidine tag at the N-terminus.
• Usage Notes: Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Mouse IFN-gamma protein in sterile ddH2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Preparation and Storage: Lyophilized Mouse IFN-gamma protein product should be stored desiccated below -20 degree C. Upon reconstitution, the protein should be stored at 4 degree C between 2-7 days and for future use below -20 degree C.; For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.; Shipping: The product is shipped at ambient temperature.

SDS-Page

(Fig.SDS-PAGE analysis of Mouse IFN-gamma protein.)

Related Product Information for IFN-gamma recombinant protein

Background: IFN gamma, also known as IFNG, is a secreted protein which belongs to the type I I interferon family. IFN gamma is produced predominantly by natural killer and natural killer T cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte effector T cells once antigen-specific immunity develops. IFN gamma has antiviral, immunoregulatory, and anti-tumor properties. IFNG, in addition to having antiviral activity, has important immunoregulatory functions, it is a potent activator of macrophages, and has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. The IFNG monomer consists of a core of six alpha-helices and an extended unfolded sequence in the C-terminal region. IFN gamma is critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. Aberrant IFN gamma expression is associated with a number of autoinflammatory and autoimmune diseases. The importance of IFN gamma in the immune system stems in part from its ability to inhibit viral replication directly, and most importantly from its immunostimulatory and immunomodulatory effects. IFNG also promotes NK cell activity.

Product Categories/Family for IFN-gamma recombinant protein

Protein

NCBI and Uniprot Product Information

• NCBI GeneID: 15978
• UniProt Accession #: P01580
• Molecular Weight: 16.9kDa
• NCBI Official Synonym Full Names: interferon gamma
• NCBI Official Symbol: Ifng
• NCBI Official Synonym Symbols: Ifg; IFN-g
• NCBI Protein Information: interferon gamma
• UniProt Protein Name: Interferon gamma
• UniProt Gene Name: Ifng
• UniProt Synonym Gene Names: IFN-gamma
• UniProt Entry Name: IFNG_MOUSE

NCBI Description

This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial infections. Mice deficient in this gene have increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. [provided by RefSeq, Dec 2015]

Uniprot Description

IFNG: Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. Homodimer. Released primarily from activated T lymphocytes. Belongs to the type II (or gamma) interferon family.

Protein type: Secreted; Cytokine; Membrane protein, integral; Secreted, signal peptide

Cellular Component: extracellular space; cell; extracellular region; intracellular; external side of plasma membrane

Molecular Function: protein binding; interferon-gamma receptor binding; cytokine activity

Biological Process: positive regulation of isotype switching to IgG isotypes; positive regulation of nitric oxide biosynthetic process; positive regulation of cell adhesion; positive regulation of osteoclast differentiation; positive regulation of interleukin-23 production; positive regulation of transcription, DNA-dependent; negative regulation of epithelial cell differentiation; positive regulation of interleukin-1 beta secretion; positive regulation of killing of cells of another organism; positive regulation of membrane protein ectodomain proteolysis; regulation of growth; positive regulation of T cell proliferation; cell cycle arrest; defense response to virus; regulation of the force of heart contraction; positive regulation of peptidyl-serine phosphorylation of STAT protein; positive regulation of synaptic transmission, cholinergic; neutrophil chemotaxis; CD8-positive, alpha-beta T cell differentiation during immune response; negative regulation of myelination; response to virus; defense response to protozoan; positive regulation of tumor necrosis factor production; inflammatory cell apoptosis; positive regulation of tyrosine phosphorylation of Stat1 protein; defense response to bacterium; neutrophil apoptosis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of interleukin-12 production; apoptosis; negative regulation of smooth muscle cell proliferation; positive regulation of interleukin-6 biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; sensory perception of mechanical stimulus; negative regulation of cell proliferation; regulation of transcription, DNA-dependent; positive regulation of MHC class II biosynthetic process; positive regulation of cell proliferation; adaptive immune response; regulation of immune response; unfolded protein response; humoral immune response; antigen processing and presentation; positive regulation of chemokine biosynthetic process; positive regulation of interleukin-12 biosynthetic process; negative regulation of interleukin-17 production; protein import into nucleus, translocation; immune response; positive regulation of neuron differentiation; regulation of insulin secretion