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SDS-Page

Interferon Alpha 5 Recombinant Protein | IFNa5 recombinant protein

Recombinant Interferon Alpha 5 (IFNa5)

Gene Names
IFNA5; INA5; INFA5; leIF G; IFN-alphaG; IFN-alpha-5
Applications
SDS-Page, Western Blot, ELISA, Immunoprecipitation
Purity
>95%
Synonyms
Interferon Alpha 5; Recombinant Interferon Alpha 5 (IFNa5); IFNa5 recombinant protein
Ordering
For Research Use Only!
Host
Host: E Coli
Source: Prokaryotic expression
Purity/Purification
>95%
Form/Format
Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Sequence
The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.
CDLPQTH NLRNKRALTL LVKMRRLSPL SCLKDRKDFG FPQEKVGAQQ IQEAQAIPVL SELTQQVLNI FTSKDSSAAW NATLLDSFCN EVHQQLNDLK ACVMQQVGVQ ESPLTQEDSLLAVRKYFHRI TVYLREKKHS PCAWEVVRAE VWRALSSSVN LLARLSKEE
Sequence Length
189
Applicable Applications for IFNa5 recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Predicted Molecular Mass
49.0kDa
Accurate Molecular Mass (KD)
49kDa
Endotoxin
<1.0EU per 1ug (determined by the LAL method)
Expression System
Prokaryotic expression
Tag
two N-terminal Tags, His-tag and GST-tag
Organism Species
Mus musculus (Mouse)
Fragment
Cys24~Glu189
Usage
Reconstitute in sterile PBS, pH7.2-pH7.4.
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

SDS-Page

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
21,942 Da
NCBI Official Full Name
interferon alpha-5
NCBI Official Synonym Full Names
interferon, alpha 5
NCBI Official Symbol
IFNA5
NCBI Official Synonym Symbols
INA5; INFA5; leIF G; IFN-alphaG; IFN-alpha-5
NCBI Protein Information
interferon alpha-5
UniProt Protein Name
Interferon alpha-5
Protein Family
UniProt Gene Name
IFNA5
UniProt Synonym Gene Names
IFN-alpha-5; LeIF G
UniProt Entry Name
IFNA5_HUMAN

Uniprot Description

IFNA5: Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase. Belongs to the alpha/beta interferon family.

Protein type: Secreted, signal peptide; Cytokine; Secreted

Chromosomal Location of Human Ortholog: 9p22

Cellular Component: extracellular space; extracellular region

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor binding; interferon-alpha/beta receptor binding; cytokine activity

Biological Process: B cell proliferation; adaptive immune response; cytokine and chemokine mediated signaling pathway; regulation of MHC class I biosynthetic process; humoral immune response; T cell activation during immune response; response to exogenous dsRNA; B cell differentiation; natural killer cell activation during immune response; innate immune response; blood coagulation; defense response to virus; positive regulation of peptidyl-serine phosphorylation of STAT protein

Research Articles on IFNa5

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Product Notes

The IFNa5 ifna5 (Catalog #AAA2028929) is a Recombinant Protein produced from Host: E Coli Source: Prokaryotic expression and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Interferon Alpha 5 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the IFNa5 ifna5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.
CDLPQTH NLRNKRALTL LVKMRRLSPL SCLKDRKDFG FPQEKVGAQQ IQEAQAIPVL SELTQQVLNI FTSKDSSAAW NATLLDSFCN EVHQQLNDLK ACVMQQVGVQ ESPLTQEDSL LAVRKYFHRI TVYLREKKHS PCAWEVVRAE VWRALSSSVN LLARLSKEE. It is sometimes possible for the material contained within the vial of "Interferon Alpha 5, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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