Bovine INTERFERON GAMMA Elisa Kit | IFN Gamma elisa kit

BOVINE INTERFERON GAMMA ELISA KIT

Cat. Num. AAA225975

• Reactivity: Bovine
• Applications: ELISA
• Synonyms: INTERFERON GAMMA; BOVINE INTERFERON GAMMA ELISA KIT; IFN Gamma; IFN Gamma elisa kit

• Reactivity: Bovine
• Specificity: IFN GAMMA

• Applicable Applications for IFN Gamma elisa kit: ELISA (EIA)
• Application Notes: Flow Cytometry: Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.; Elisa: This assay is based on a sandwich ELISA using two different mouse anti bovine IFN-gamma monoclonal antibodies and a recombinant bovine IFN-gamma as a standard.
• Reagents in the Kit: 96 well microtiter plates x 5; Microplate adhesive sealers x 5; Coating antibody - 1vial ; Coating Buffer - 1 vial (5 x concentrated); Detection antibody - Biotin conjugated - 1 vial ; Streptavidin: HRP conjugate - 1 vial; TMB Substrate 1 vial; Bovine IFN gamma standard (400ng/ml - Lyophilized) 1 vial
• Intended Use: Bovine Interferon gamma ELISA KIt is an ELISA kit (5 x 96 wells) designed for the quantitive determination of bovine interferon gamma (BoIFN-gamma) in bovine serum, plasma and culture supernatant.; This assay can measure BoIFN-gamma in the range 0.025-50 ng/ml in bovine serum, plasma and culture supernatant. Detailed instructions for use of the kit can be found here.; The following additional reagents and Equipment are also required for successful use of this kits BUT ARE NOT CONTAINED WITHIN THIS KIT; Wash buffer (see kit insert for recipe); Blocking solution (or see kit insert for recipe); Stop Solution (see kit insert for recipe); Distilled or Deionised water; Plate reader with 450nm reading capacity; Pipettes for dispensing up to 250ul; Tubes for preparing standard and sample dilutions.
• Preparation and Storage: Prior to use store all kit components at 4 degree C. DO NOT FREEZE. Please view the handbook for further details.; Storage in frost free freezers is not recommended. This Kit contains components that are photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature components within this kit.; The contents of this kit should be stored undiluted. Should components within this kit contain a precipitate we recommend microcentrifugation before use.

Testing Data

(Typical assay curve using standards and reagents supplied in the Bovine IFN gamma ELISA kit)

Related Product Information for IFN Gamma elisa kit

The Bovine Interferon Gamma Kit is specific for bovine interferon gamma (IFN-gamma), a secreted cytokine involved in anti-viral activity and growth regulation belonging to the type II interferon family.
IFN-gamma is produced by lymphocytes in response to activation by specific antigens or mitogens. In addition to its anti-viral activity, IFN-gamma has important immunoregulatory functions in the activation of macrophages and anti-proliferative effects upon transformed cells.