Immunofluorescence (IF)
(Immunofluorescence analysis of methanol-fixed HeLa, using Protease Inhibitor 15 antibody at 1:500 dilution.)
Rabbit Protease Inhibitor 15 Polyclonal Antibody | anti-PI15 antibody
Protease Inhibitor 15 antibody
Gene Names
PI15; P24TI; P25TI; CRISP8
Applications
Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Protease Inhibitor 15 antibody was purified by antigen-affinity chromatography
Synonyms
Protease Inhibitor 15; Polyclonal Antibody; Protease Inhibitor 15 antibody; Polyclonal Protease Inhibitor 15; Anti-Protease Inhibitor 15; P24TI; 25 kDa trypsin inhibitor; protease inhibitor 15; P25TI; DKFZp686F0366; sugarCrisp; PI15; CRISP8; CRISP-8; Protease Inhibitor -15; anti-PI15 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Protease Inhibitor 15 antibody was purified by antigen-affinity chromatography
Form/Format
Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration
0.6 mg/ml (varies by lot)
Sequence Length
258
Applicable Applications for anti-PI15 antibody
Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes
IF:1:100-1:500
ICC:1:100-1:500
IHC-P: 1:100-1:500
WB: 1:500-1:3000
ICC:1:100-1:500
IHC-P: 1:100-1:500
WB: 1:500-1:3000
Biological Significance
This gene encodes a trypsin inhibitor. The protein shares similarity to insect venom allergens, mammalian testis-specific proteins and plant pathogenesis-related proteins. It is frequently expressed in human neuroblastoma and glioblastoma cell lines, and thus may play a role in the central nervous system.
Cross-Reactivity
Human
Immunogen
Protease Inhibitor 15 antibody was raised in rabbit using a synthetic peptide contain a sequence corresponding to a region within amino acids 195 and 208 of Protease Inhibitor 15
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Immunofluorescence (IF)
(Immunofluorescence analysis of methanol-fixed HeLa, using Protease Inhibitor 15 antibody at 1:500 dilution.)
Immunofluorescence (IF)
(Immunofluorescence analysis of methanol-fixed HeLa, using Protease Inhibitor 15 antibody at 1:500 dilution.)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A: Hela) using a 12 % SDS PAGE gel and Protease Inhibitor 15 antibody at a dilution of 1:1000)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A: Hela) using a 12 % SDS PAGE gel and Protease Inhibitor 15 antibody at a dilution of 1:1000)
Immunohistochemistry (IHC)
(Immunohistochemical staining of paraffin-embedded Breast ca using Protease Inhibitor 15 antibody at a dilution of 1:250)
Immunohistochemistry (IHC)
(Immunohistochemical staining of paraffin-embedded Breast ca using Protease Inhibitor 15 antibody at a dilution of 1:250)
Related Product Information for anti-PI15 antibody
Affinity purified Rabbit polyclonal Protease Inhibitor 15 antibody
Product Categories/Family for anti-PI15 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
29 kDa
NCBI Official Full Name
peptidase inhibitor 15 preproprotein
NCBI Official Synonym Full Names
peptidase inhibitor 15
NCBI Official Symbol
PI15
NCBI Official Synonym Symbols
P24TI; P25TI; CRISP8
NCBI Protein Information
peptidase inhibitor 15
UniProt Protein Name
Peptidase inhibitor 15
Protein Family
UniProt Gene Name
PI15
UniProt Synonym Gene Names
CRISP8; P25TI; PI-15; p25TI; CRISP-8
UniProt Entry Name
PI15_HUMAN
NCBI Description
This gene encodes a trypsin inhibitor. The protein shares similarity to insect venom allergens, mammalian testis-specific proteins and plant pathogenesis-related proteins. It is frequently expressed in human neuroblastoma and glioblastoma cell lines, and thus may play a role in the central nervous system. [provided by RefSeq, Jul 2008]
Uniprot Description
PI15: Serine protease inhibitor which displays weak inhibitory activity against trypsin. Belongs to the CRISP family.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 8q21.11
Molecular Function: protease inhibitor activity
Biological Process: negative regulation of peptidase activity
Similar Products
Product Notes
The PI15 pi15 (Catalog #AAA835109) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Protease Inhibitor 15 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB). IF:1:100-1:500 ICC:1:100-1:500 IHC-P: 1:100-1:500 WB: 1:500-1:3000. Researchers should empirically determine the suitability of the PI15 pi15 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Protease Inhibitor 15, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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