IFIT3 recombinant protein

IFIT3, 1-490aa, Human, His tag, E Coli

Cat. Num. AAA206045

• Gene Names: IFIT3; P60; IRG2; IFI60; IFIT4; ISG60; RIG-G; cig41; CIG-49; GARG-49
• Applications: SDS-Page
• Purity: > 80% by SDS-PAGE
• Synonyms: IFIT3; 1-490aa; Human; His tag; E Coli; Interferon-induced protein with tetratricopeptiede repeats 3 isoform a; CIG-49; GARG-49; IFI60; IFIT4; IRG2; ISG60; P60; RIG-G; IFIT3 recombinant protein

• Host: E Coli
• Purity/Purification: > 80% by SDS-PAGE
• Form/Format: Liquid. In phosphate buffered saline (pH 7.4) containing 20% glycerol, 1mM DTT
• Concentration: 1mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMSEVTKN SLEKILPQLK CHFTWNLFKE DSVSRDLEDR VCNQIEFLNT EFKATMYNLL AYIKHLDGNN EAALECLRQA EELIQQEHAD QAEIRSLVTW GNYAWVYYHL GRLSDAQIYV DKVKQTCKKF SNPYSIEYSE LDCEEGWTQL KCGRNERAKV CFEKALEEKP NNPEFSSGLA IAMYHLDNHP EKQFSTDVLK QAIELSPDNQ YVKVLLGLKL QKMNKEAEGE QFVEEALEKS PCQTDVLRSA AKFYRRKGDL DKAIELFQRV LESTPNNGYL YHQIGCCYKA KVRQMQNTGE SEASGNKEMI EALKQYAMDY SNKALEKGLN PLNAYSDLAE FLETECYQTP FNKEVPDAEK QQSHQRYCNL QKYNGKSEDT AVQHGLEGLS ISKKSTDKEE IKDQPQNVSE NLLPQNAPNY WYLQGLIHKQ NGDLLQAAKC YEKELGRLLR DAPSGIGSIF LSASELEDGS EEMGQGAVSS SPRELLSNSE QLN
• Sequence Length: 490

• Applicable Applications for IFIT3 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for IFIT3 recombinant protein

IFIT3 also known as Interferon-induced protein with tetratricopeptiede repeats 3 isoform a. IFN-induced antiviral protein which acts as an inhibitor of cellular as well as viral processes, cell migration, proliferation, signaling, and viral replication. IFIT3 is significantly induced upon RNA virus infection. Ectopic expression or knockdown of IFIT3 could, respectively, enhance or impair IRF3-mediated gene expression. Recombinant human IFIT3 was expressed in E Coli and purified by using conventional chromatography techniques

Product Categories/Family for IFIT3 recombinant protein

Immunology

References

Fensterl V., et al. (2011) J. Interferon Cytokine Res. 31:71-78.; Liu X.Y., et al. (2011) J. Immunol. 187:2559-2568.;

NCBI and Uniprot Product Information

• NCBI GI #: 31542980
• NCBI GeneID: 3437
• NCBI Accession #: NP_001540
• NCBI GenBank Nucleotide #: NM_001549.5
• UniProt Accession #: O14879; Q99634; Q9BSK7
• Molecular Weight: 58.4kDa (513aa)
• NCBI Official Full Name: interferon-induced protein with tetratricopeptide repeats 3 isoform a
• NCBI Official Synonym Full Names: interferon induced protein with tetratricopeptide repeats 3
• NCBI Official Symbol: IFIT3
• NCBI Official Synonym Symbols: P60; IRG2; IFI60; IFIT4; ISG60; RIG-G; cig41; CIG-49; GARG-49
• NCBI Protein Information: interferon-induced protein with tetratricopeptide repeats 3
• UniProt Protein Name: Interferon-induced protein with tetratricopeptide repeats 3
• Protein Family: Interferon-induced protein with tetratricopeptide repeats
• UniProt Gene Name: IFIT3
• UniProt Synonym Gene Names: CIG-49; IFI60; IFIT4; ISG60; IFIT-3; IFI-60K; IFIT-4; P60; RIG-G

Uniprot Description

IFN-induced antiviral protein which acts as an inhibitor of cellular as well as viral processes, cell migration, proliferation, signaling, and viral replication. Enhances MAVS-mediated host antiviral responses by serving as an adapter bridging TBK1 to MAVS which leads to the activation of TBK1 and phosphorylation of IRF3 and phosphorylated IRF3 translocates into nucleus to promote antiviral gene transcription. Exihibits an antiproliferative activity via the up-regulation of cell cycle negative regulators CDKN1A/p21 and CDKN1B/p27. Normally, CDKN1B/p27 turnover is regulated by COPS5, which binds CDKN1B/p27 in the nucleus and exports it to the cytoplasm for ubiquitin-dependent degradation. IFIT3 sequesters COPS5 in the cytoplasm, thereby increasing nuclear CDKN1B/p27 protein levels. Upregulates CDKN1A/p21 by downregulating MYC, a repressor of CDKN1A/p21. Can negatively regulate the apoptotic effects of IFIT2.