Human iduronate 2-sulfatase ELISA Kit | IDS elisa kit

Human iduronate sulfatase, IDS ELISA Kit

Cat. Num. AAA705044

• Gene Names: IDS; MPS2; SIDS
• Reactivity: Human
• Synonyms: iduronate 2-sulfatase; Human iduronate sulfatase; IDS ELISA Kit; MPS2; SIDS; alpha-L-iduronate sulfate sulfatase; iduronate 2-sulfatase 14 kDa chain; iduronate 2-sulfatase 42 kDa chain; iduronate-2-sulfatase; idursulfase; IDS elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human IDES. No significant cross-reactivity or interference between human IDES and analogues was observed.
• Sequence Length: 550

• Samples: Serum, Plasma, Urine And Tissue Homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 47 pg/ml-3000 pg/ml
• Sensitivity: 11.75 pg/ml.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IDS elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IDES has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IDES present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IDES is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IDES bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 4557659
• NCBI GeneID: 3423
• NCBI Accession #: NP_000193.1
• NCBI GenBank Nucleotide #: NM_000202.6
• UniProt Accession #: P22304; Q14604; Q9BRM3; D3DWT4
• Molecular Weight: 61,873 Da
• NCBI Official Full Name: iduronate 2-sulfatase isoform a preproprotein
• NCBI Official Synonym Full Names: iduronate 2-sulfatase
• NCBI Official Symbol: IDS
• NCBI Official Synonym Symbols: MPS2; SIDS
• NCBI Protein Information: iduronate 2-sulfatase; idursulfase; iduronate 2-sulfatase 14 kDa chain; iduronate 2-sulfatase 42 kDa chain; alpha-L-iduronate sulfate sulfatase
• UniProt Protein Name: Iduronate 2-sulfatase
• Protein Family: Iduronate 2-sulfatase
• UniProt Gene Name: IDS
• UniProt Synonym Gene Names: SIDS; Idursulfase
• UniProt Entry Name: IDS_HUMAN

NCBI Description

The protein encoded by this gene belongs to the sulfatase family, is localized to the lysosome, and is involved in lysosomal degradation of heparan sulfate and dermatan sulfate. Mutations in this gene are associated with the X-linked lysosomal storage disease, mucopolysaccharidosis type II, also known as Hunter syndrome. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Aug 2013]

Uniprot Description

Function: Required for the lysosomal degradation of heparan sulfate and dermatan sulfate.

Catalytic activity: Hydrolysis of the 2-sulfate groups of the L-iduronate 2-sulfate units of dermatan sulfate, heparan sulfate and heparin.

Cofactor: Binds 1 calcium ion per subunit