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Gene Sequencing (Extract)

Intercellular Adhesion Molecule 5 Recombinant Protein | ICAM5 recombinant protein

Recombinant Intercellular Adhesion Molecule 5 (ICAM5)

Gene Names
Icam5; TLN; CD50; Tlcn; Icam3; ICAM-3
Reactivity
Mus musculus (Mouse)
Applications
SDS-Page, Western Blot
Purity
> 95%
Synonyms
Intercellular Adhesion Molecule 5; Recombinant Intercellular Adhesion Molecule 5 (ICAM5); ICAM5 recombinant protein
Ordering
For Research Use Only!
Host
E.coli
Reactivity
Mus musculus (Mouse)
Purity/Purification
> 95%
Form/Format
100mM NaHCO3, 500mM NaCl, pH8.3, containing 0.01% SKL, 5% Trehalose.
Concentration
Original Concentration: 400ug/mL (varies by lot)
Sequence
LIRRSFV GEPPRARGAM LTARVLARRE DHRVNFSCLA ELDLRPHGLG LFANSSAPRQ LRTFAMPPHS PSLIAPRVLE VDSERPVTCT LDGLFPAPEA GVYLSLGDQR LNPNVTLDGD SLVATATATA SAEQEGTKQL MCVVTLGGES RETQENLTVY SFPTPLLTLS EPEAPEGKMV TISCWAGARA LVTLEGIPAA VPGQPAELQL NVTKNDDKRG FFCDAALDVD GETLRKNQSS ELRVLYAPRL DDLDCPRSWTWPEGPEQTLH CEARGNPEPS V
Applicable Applications for ICAM5 recombinant protein
Positive Control; Immunogen; SDS-PAGE; Western Blot (WB).
Application Notes
(May be suitable for use in other assays to be determined by the end user.)
Source
Prokaryotic expression
Residues
Leu164~Val441
Tags
N-terminal His Tag
Subcellular Location
Membrane
Traits
Freeze-dried powder
Predicted isoelectric point
4.7
Phenomenon explanation
The possible reasons that the actual band size differs from the predicted are as follows:
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affect the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
Usage
Reconstitute in ddH2O to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.

Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Gene Sequencing (Extract)

Gene Sequencing (Extract)

SDS-Page

SDS-Page

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
N/APredicted Molecular Mass: 33.8kDa
Accurate Molecular Mass: 38kDa as determined by SDS-PAGE reducing conditions.
NCBI Official Full Name
intercellular adhesion molecule 5
NCBI Official Synonym Full Names
intercellular adhesion molecule 5, telencephalin
NCBI Official Symbol
Icam5
NCBI Official Synonym Symbols
TLN; CD50; Tlcn; Icam3; ICAM-3
NCBI Protein Information
intercellular adhesion molecule 5
UniProt Protein Name
Intercellular adhesion molecule 5
UniProt Gene Name
Icam5
UniProt Synonym Gene Names
Icam3; Tlcn; ICAM-5
UniProt Entry Name
ICAM5_MOUSE

Uniprot Description

ICAM5: ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). Belongs to the immunoglobulin superfamily. ICAM family.

Protein type: Membrane protein, integral; Cell adhesion

Cellular Component: membrane; integral to plasma membrane; integral to membrane; plasma membrane

Molecular Function: integrin binding; protein binding

Biological Process: cell-cell adhesion; cellular response to stimulus; cell adhesion; phagocytosis

Research Articles on ICAM5

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Product Notes

The ICAM5 icam5 (Catalog #AAA2028857) is a Recombinant Protein produced from E.coli and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Intercellular Adhesion Molecule 5 (ICAM5) reacts with Mus musculus (Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's Intercellular Adhesion Molecule 5 can be used in a range of immunoassay formats including, but not limited to, Positive Control; Immunogen; SDS-PAGE; Western Blot (WB). (May be suitable for use in other assays to be determined by the end user.). Researchers should empirically determine the suitability of the ICAM5 icam5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: LIRRSFV GEPPRARGAM LTARVLARRE DHRVNFSCLA ELDLRPHGLG LFANSSAPRQ LRTFAMPPHS PSLIAPRVLE VDSERPVTCT LDGLFPAPEA GVYLSLGDQR LNPNVTLDGD SLVATATATA SAEQEGTKQL MCVVTLGGES RETQENLTVY SFPTPLLTLS EPEAPEGKMV TISCWAGARA LVTLEGIPAA VPGQPAELQL NVTKNDDKRG FFCDAALDVD GETLRKNQSS ELRVLYAPRL DDLDCPRSWT WPEGPEQTLH CEARGNPEPS V. It is sometimes possible for the material contained within the vial of "Intercellular Adhesion Molecule 5, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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