Sheep Intercellular Adhesion Molecule 1 ELISA Kit | ICAM-1 elisa kit

Sheep Intercellular Adhesion Molecule 1 ELISA Kit

Cat. Num. AAA21511

• Gene Names: ICAM1; BB2; CD54; P3.58
• Reactivity: Sheep
• Synonyms: Intercellular Adhesion Molecule 1; Sheep Intercellular Adhesion Molecule 1 ELISA Kit; ICAM-1 elisa kit

• Reactivity: Sheep
• Specificity: No significant cross-reactivity or interference between Sheep ICAM-1 and analogues was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine
• Assay Type: Sandwich
• Detection Range: 6.25ng/ml-200ng/ml.
• Sensitivity: 1.0ng/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated ICAM-1 concentrations in Sheep serum, plasma and other body fluids. Using Purified Sheep ICAM-1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add ICAM-1 and ICAM-1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of ICAM-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for ICAM-1 elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated ICAM-1 concentrations in Sheep serum, plasma and other body fluids. Using Purified Sheep ICAM-1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add ICAM-1 and ICAM-1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of ICAM-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 167466198
• NCBI GeneID: 3383
• NCBI Accession #: NP_000192.2
• NCBI GenBank Nucleotide #: NM_000201.2
• UniProt Accession #: P05362; Q5NKV7; Q96B50; B2R6M3
• Molecular Weight: 57,825 Da
• NCBI Official Full Name: intercellular adhesion molecule 1
• NCBI Official Synonym Full Names: intercellular adhesion molecule 1
• NCBI Official Symbol: ICAM1
• NCBI Official Synonym Symbols: BB2; CD54; P3.58
• NCBI Protein Information: intercellular adhesion molecule 1; ICAM-1; cell surface glycoprotein P3.58; major group rhinovirus receptor; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor
• UniProt Protein Name: Intercellular adhesion molecule 1
• Protein Family: Intercellular adhesion molecule
• UniProt Gene Name: ICAM1
• UniProt Synonym Gene Names: ICAM-1
• UniProt Entry Name: ICAM1_HUMAN

NCBI Description

This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. [provided by RefSeq, Jul 2008]

Uniprot Description

ICAM1: a type I membrane protein of the immunoglobulin superfamily. Is a ligand for the leukocyte adhesion LFA-1 protein (Integrin alpha-L/beta-2) and a Rhinovirus receptor. Typically expressed on endothelial cells and cells of the immune system. ICAM1 binds to integrins of type CD11a / CD18, or CD11b / CD18. Its expression is activated by p53 in an NF-kappaB-independent manner. Induced by TNFalpha in a process that involves IKKbeta.

Protein type: Immunoglobulin superfamily; Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19p13.3-p13.2

Cellular Component: extracellular space; focal adhesion; cell surface; membrane; integral to plasma membrane; plasma membrane; immunological synapse; external side of plasma membrane

Molecular Function: viral receptor activity; integrin binding; protein binding; transmembrane receptor activity; receptor activity

Biological Process: extracellular matrix organization and biogenesis; entry of virus into host cell; positive regulation of nitric oxide biosynthetic process; T cell antigen processing and presentation; response to organic cyclic substance; activation of NF-kappaB transcription factor; positive regulation of cellular extravasation; regulation of cell shape; leukocyte adhesion; cellular response to nutrient levels; sensory perception of sound; ovarian follicle development; T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell; membrane to membrane docking; response to sulfur dioxide; cell adhesion; regulation of leukocyte mediated cytotoxicity; acute inflammatory response to antigenic stimulus; response to drug; regulation of cell adhesion; virion attachment, binding of host cell surface receptor; regulation of immune response; negative regulation of calcium ion transport; cytokine and chemokine mediated signaling pathway; response to amphetamine; cell aging; response to amino acid stimulus; heterophilic cell adhesion; positive regulation of peptidyl-tyrosine phosphorylation; response to ethanol; positive regulation of actin filament polymerization; response to copper ion; positive regulation of vasoconstriction; cell adhesion mediated by integrin; adhesion to host; response to ionizing radiation; leukocyte migration

Disease: Malaria, Susceptibility To