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Western Blot (WB) (Western Blot analysis of HepG2 cells using DDX8 Polyclonal Antibody)

Rabbit anti-Human, Mouse DHX8 Polyclonal Antibody | anti-DHX8 antibody

DHX8 Antibody

Gene Names
DHX8; DDX8; HRH1; PRP22; PRPF22
Reactivity
Human, Mouse
Applications
Western Blot, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
DHX8; Polyclonal Antibody; DHX8 Antibody; DDX8; ATP-dependent RNA helicase DHX8; DEAH box protein 8; RNA helicase HRH1; anti-DHX8 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Liquid; In Phosphate Buffered Saline, 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Applicable Applications for anti-DHX8 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
ELISA: 1:5000
Immunogen
Synthesized peptide derived from the Internal region of human DDX8.
Immunogen Species
Human
Conjugate
Non-conjugated
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western Blot analysis of HepG2 cells using DDX8 Polyclonal Antibody)

Western Blot (WB) (Western Blot analysis of HepG2 cells using DDX8 Polyclonal Antibody)
Product Categories/Family for anti-DHX8 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
139,315 Da
NCBI Official Full Name
ATP-dependent RNA helicase DHX8
NCBI Official Synonym Full Names
DEAH (Asp-Glu-Ala-His) box polypeptide 8
NCBI Official Symbol
DHX8
NCBI Official Synonym Symbols
DDX8; HRH1; PRP22; PRPF22
NCBI Protein Information
ATP-dependent RNA helicase DHX8; RNA helicase HRH1; DEAH box protein 8; DEAH-box protein 8; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 8 (RNA helicase)
UniProt Protein Name
ATP-dependent RNA helicase DHX8
UniProt Gene Name
DHX8
UniProt Synonym Gene Names
DDX8
UniProt Entry Name
DHX8_HUMAN

NCBI Description

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is highly homologous to yeast Prp22. This protein facilitates nuclear export of spliced mRNA by releasing the RNA from the spliceosome. [provided by RefSeq, Jul 2008]

Uniprot Description

DDX8: Facilitates nuclear export of spliced mRNA by releasing the RNA from the spliceosome. Belongs to the DEAD box helicase family. DEAH subfamily. DDX8/PRP22 sub-subfamily.

Protein type: RNA-binding; RNA processing; RNA splicing; EC 3.6.4.13; Helicase; Spliceosome

Chromosomal Location of Human Ortholog: 17q21.31

Cellular Component: nucleoplasm; spliceosome; cytoplasm; nucleus

Molecular Function: identical protein binding; protein binding; ATP binding; ATP-dependent RNA helicase activity

Biological Process: RNA processing; nuclear mRNA splicing, via spliceosome; RNA splicing

Research Articles on DHX8

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Product Notes

The DHX8 dhx8 (Catalog #AAA7116229) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DHX8 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's DHX8 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500-1:2000 ELISA: 1:5000. Researchers should empirically determine the suitability of the DHX8 dhx8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DHX8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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