Monkey Islet amyloid polypeptide ELISA Kit | IAPP elisa kit

Monkey Islet amyloid polypeptide ELISA Kit

Cat. Num. AAA740873

• Gene Names: IAPP; DAP; IAP
• Reactivity: Monkey
• Synonyms: Islet amyloid polypeptide; Monkey Islet amyloid polypeptide ELISA Kit; IAPP elisa kit

• Reactivity: Monkey

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 25-500pg/mL
• Sensitivity: 1.0pg/mL
• Intended Uses: This IAPP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey IAPP. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IAPP elisa kit

Principle of the assay: IAPP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IAPP antibody and an IAPP-HRP conjugate. The assay sample and buffer are incubated together with IAPP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IAPP concentration since IAPP from samples and IAPP-HRP conjugate compete for the anti-IAPP antibody binding site. Since the number of sites is limited, as more sites are occupied by IAPP from the sample, fewer sites are left to bind IAPP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IAPP concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4557655
• NCBI GeneID: 3375
• NCBI Accession #: NP_000406.1
• NCBI GenBank Nucleotide #: NM_000415.2
• Molecular Weight: 9,806 Da
• NCBI Official Full Name: islet amyloid polypeptide
• NCBI Official Synonym Full Names: islet amyloid polypeptide
• NCBI Official Symbol: IAPP
• NCBI Official Synonym Symbols: DAP; IAP
• NCBI Protein Information: islet amyloid polypeptide; amylin; insulinoma amyloid peptide; diabetes-associated peptide; Islet amyloid polypeptide (diabetes-associated peptide; amylin)
• UniProt Protein Name: Islet amyloid polypeptide
• Protein Family: Islet amyloid polypeptide
• UniProt Gene Name: IAPP
• UniProt Synonym Gene Names: DAP
• UniProt Entry Name: IAPP_HUMAN

NCBI Description

Islet, or insulinoma, amyloid polypeptide is commonly found in pancreatic islets of patients suffering diabetes mellitus type II, or harboring an insulinoma. While the association of amylin with the development of type II diabetes has been known for some time, a direct causative role for amylin has been harder to establish. Studies suggest that amylin, like the related beta-amyloid (Abeta) associated with Alzheimer's disease, can induce apoptotic cell-death in particular cultured cells, an effect that may be relevant to the development of type II diabetes. [provided by RefSeq, Apr 2011]

Uniprot Description

IAPP: Selectively inhibits insulin-stimulated glucose utilization and glycogen deposition in muscle, while not affecting adipocyte glucose metabolism. Belongs to the calcitonin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 12p12.1

Cellular Component: extracellular space; cell soma; extracellular region

Molecular Function: identical protein binding; hormone activity; receptor binding

Biological Process: negative regulation of cell differentiation; cell-cell signaling; eating behavior; apoptosis; negative regulation of bone resorption; signal transduction; sensory perception of pain; endocrine pancreas development