Transferrin Peptide | TF peptide

Transferrin, Human (Tf) Control Peptide

Cat. Num. AAA659562

• Gene Names: TF; TFQTL1; PRO1557; PRO2086
• Applications: ELISA, Western Blot
• Purity: Highly Purified; ~98%
• Synonyms: Transferrin; Human (Tf) Control Peptide; TF peptide

• Host: Human serum
• Purity/Purification: Highly Purified; ~98%
• Form/Format: Supplied in 100 uL denaturing SDS-PAGE sample buffer.

• Applicable Applications for TF peptide: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot and ELISA.; Dilution: Western Blot: Ready to use (10 ul/lane); ELISA: Coat ELISA plates at 1ug/ml
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Testing Data

(Transferin, TF, Apotransferrin, Beta-1 Metal-binding Globulin, DKFZp781D0156, PRO1400, PRO1557, PRO2086, Serotransferrin Precursor, Siderophilin)

Related Product Information for TF peptide

Elemental iron is required for a variety of normal cellular functions and vital for proper growth and development. However, natural iron is quite insoluble and excess iron is harmful, since it can catalyze the formation of potentially damaging reactive oxygen species. The major pool of body iron (~85%; 40-50mg/kg) is found in circulating hemoglobin and muscle myoglobin. Iron absorption occurs primarily in the intestine (duodenum) and inversely related to body iron reserve. Several proteins including Ferritin, transferrin (Tf), transferrin receptors (TfRs), and iron regulatory proteins (IRPs) etc play a key role in iron metabolism.

Transferrin (Tf, human chromosome 3, 679 aa), a serum glycoprotein of ~80kD and synthesized in the liver, is the primary protein of inter-organ transport of nonheme iron. Tf can bind two iron atoms. Tf binds to membrane Transferrin receptors (TfRs) and taken up by endocytosis. Iron is released from Tf, within acidic endosomes, into the cytoplasm apparently through the action of DMT1. The apoTf-TfR complex is returned to the cell surface, where, apo-Tf dissociates from TfR at the extracellular pH. The classical TfR, now termed TfR1, is a homodimeric (95kD subunits) type II membrane glycoprotein that binds two molecules of Tf. Human TfR1 (human 760 aa; mouse 763 aa) has a cytoplasmic domain 1-67aa, 68-88 aa TM, and 89-760 aa as extracellular domains. A monomeric serum form or soluble TfR1 (~80kD) also exists that lacks residues 1-100 aa. Recently, a second Tf receptor, TfR2, has been cloned and characterized. TfR2 shares 45% identity with TfR1, and 27% with PMSA. Several variants of Tf have been identified with varying iron binding ability.

Product Categories/Family for TF peptide

Molecular Biology; MB-Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 339453
• NCBI GeneID: 7018
• UniProt Accession #: P02787; O43890; Q1HBA5; Q9NQB8; Q9UHV0
• Molecular Weight: 77,064 Da
• NCBI Official Full Name: transferrin
• NCBI Official Synonym Full Names: transferrin
• NCBI Official Symbol: TF
• NCBI Official Synonym Symbols: TFQTL1; PRO1557; PRO2086
• NCBI Protein Information: serotransferrin; siderophilin; beta-1 metal-binding globulin
• UniProt Protein Name: Serotransferrin
• Protein Family: Transferrin
• UniProt Gene Name: TF
• UniProt Synonym Gene Names: Transferrin
• UniProt Entry Name: TRFE_HUMAN

NCBI Description

This gene encodes a glycoprotein with an approximate molecular weight of 76.5 kDa. It is thought to have been created as a result of an ancient gene duplication event that led to generation of homologous C and N-terminal domains each of which binds one ion of ferric iron. The function of this protein is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body. This protein may also have a physiologic role as granulocyte/pollen-binding protein (GPBP) involved in the removal of certain organic matter and allergens from serum. [provided by RefSeq, Sep 2009]

Uniprot Description

Function: Transferrins are iron binding transport proteins which can bind two Fe3+ ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.

Subunit structure: Monomer.

Subcellular location: Secreted.

Tissue specificity: Expressed by the liver and secreted in plasma.

Polymorphism: Different polymorphic variants of transferrin are known. The sequence shown is the predominant electrophoretic variant (C1 or TF*C1).

Involvement in disease: Atransferrinemia (ATRAF) [MIM:209300]: A rare autosomal recessive disorder characterized by abnormal synthesis of transferrin leading to iron overload and microcytic hypochromic anemia.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.4 Ref.37

Sequence similarities: Belongs to the transferrin family.Contains 2 transferrin-like domains.

Sequence caution: The sequence AAF22007.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.