Human Uroporphyrinogen III Synthase (UROS) RTU ELISA Kit | UROS rtu elisa kit

Human Uroporphyrinogen III Synthase (UROS) ELISA Kit

Cat. Num. AAA4503240

• Gene Names: UROS; UROIIIS
• Reactivity: Human
• Synonyms: Uroporphyrinogen III Synthase (UROS); Human Uroporphyrinogen III Synthase (UROS) ELISA Kit; UROIIIS; Hydroxymethylbilane hydrolyase; Uroporphyrinogen-III cosynthase; UROS rtu elisa kit

• Reactivity: Human
• Sequence Length: 265

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates or Other Biological Fluids.
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.114ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for UROS rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to UROS. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to UROS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain UROS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of UROS in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4557873
• NCBI GeneID: 7390
• NCBI Accession #: NP_000366.1
• NCBI GenBank Nucleotide #: NM_000375.2
• UniProt Accession #: P10746; Q9H2T1; B2RC13; D3DRF7
• Molecular Weight: 28,628 Da
• NCBI Official Full Name: uroporphyrinogen-III synthase isoform 2
• NCBI Official Synonym Full Names: uroporphyrinogen III synthase
• NCBI Official Symbol: UROS
• NCBI Official Synonym Symbols: UROIIIS
• NCBI Protein Information: uroporphyrinogen-III synthase
• UniProt Protein Name: Uroporphyrinogen-III synthase
• Protein Family: Uroporphyrinogen-III synthase
• UniProt Gene Name: UROS
• UniProt Synonym Gene Names: UROIIIS; UROS

NCBI Description

The protein encoded by this gene catalyzes the fourth step of porphyrin biosynthesis in the heme biosynthetic pathway. Defects in this gene cause congenital erythropoietic porphyria (Gunther's disease). [provided by RefSeq, Jul 2008]

Uniprot Description

Catalyzes cyclization of the linear tetrapyrrole, hydroxymethylbilane, to the macrocyclic uroporphyrinogen III, the branch point for the various sub-pathways leading to the wide diversity of porphyrins. Porphyrins act as cofactors for a multitude of enzymes that perform a variety of processes within the cell such as methionine synthesis (vitamin B12) or oxygen transport (heme).CautionVariant Ala-62 was originally reported to result in no detectable enzyme activity (PubMed:1737856), as measured in recombinant crude lysate extracts. Further experiments with the purified enzyme have shown that this variant does not affect activity (PubMed:11689424).