Rat Transferrin Receptor (TFR) RTU ELISA Kit | TFR rtu elisa kit

Rat Transferrin Receptor (TFR) ELISA Kit

Cat. Num. AAA4502798

• Gene Names: Tfrc; Trfr
• Reactivity: Rat
• Synonyms: Transferrin Receptor (TFR); Rat Transferrin Receptor (TFR) ELISA Kit; CD71; TFR1; P90; TFRC; T9; Trfr; sTfR; Transferrin receptor protein 1; serum form; TFR rtu elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of TFR. No significant cross-reactivity or interference between TFR and analogues was observed.
• Sequence Length: 622

• Samples: Human Serum, Plasma, Tissue Homogenates, Cell Lysates Or Other Biological Fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 1.56-100ng/mL
• Sensitivity: 0.58ng/mL.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TFR were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TFR were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TFR rtu elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of TFR in human serum, plasma, tissue homogenates, cell lysates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TFR. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to TFR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TFR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of TFR in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 20140355
• NCBI GeneID: 64678
• UniProt Accession #: Q99376
• Molecular Weight: 70,153 Da
• NCBI Official Full Name: Transferrin receptor protein 1
• NCBI Official Synonym Full Names: transferrin receptor
• NCBI Official Symbol: Tfrc
• NCBI Official Synonym Symbols: Trfr
• NCBI Protein Information: transferrin receptor protein 1
• UniProt Protein Name: Transferrin receptor protein 1
• UniProt Gene Name: Tfrc
• UniProt Synonym Gene Names: Trfr; TR; TfR; TfR1; Trfr

NCBI Description

receptor for transferrin; involved in regulating stellate cell activation [RGD, Feb 2006]

Uniprot Description

Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (). Positively regulates T and B cell proliferation through iron uptake ().