Human Thymidylate Synthetase (TYMS) RTU ELISA Kit | TYMS rtu elisa kit

Human Thymidylate Synthetase (TYMS) ELISA Kit

Cat. Num. AAA4503167

• Gene Names: TYMS; TS; TMS; HST422
• Reactivity: Human
• Synonyms: Thymidylate Synthetase (TYMS); Human Thymidylate Synthetase (TYMS) ELISA Kit; TS; TMS; TSase; Thymidylate Synthase; TYMS rtu elisa kit

• Reactivity: Human
• Sequence Length: 313

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates or Other Biological Fluids.
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.128ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TYMS rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TYMS. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to TYMS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TYMS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of TYMS in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4507751
• NCBI GeneID: 7298
• NCBI Accession #: NP_001062.1
• NCBI GenBank Nucleotide #: NM_001071.2
• UniProt Accession #: P04818; Q8WYK3; Q8WYK4
• Molecular Weight: 26,140 Da
• NCBI Official Full Name: thymidylate synthase
• NCBI Official Synonym Full Names: thymidylate synthetase
• NCBI Official Symbol: TYMS
• NCBI Official Synonym Symbols: TS; TMS; HST422
• NCBI Protein Information: thymidylate synthase
• UniProt Protein Name: Thymidylate synthase
• UniProt Gene Name: TYMS
• UniProt Synonym Gene Names: TS; TS; TSase

NCBI Description

Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using, 10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occurring antisense transcript, mitochondrial enolase superfamily member 1 (GeneID:55556), vary inversely when cell-growth progresses from late-log to plateau phase. Polymorphisms in this gene may be associated with etiology of neoplasia, including breast cancer, and response to chemotherapy. [provided by RefSeq, Aug 2017]

Uniprot Description

Contributes to the de novo mitochondrial thymidylate biosynthesis pathway.