Human Testis Anion Transporter 1 (TAT1) RTU ELISA Kit | TAT1 rtu elisa kit

Human Testis Anion Transporter 1 (TAT1) ELISA Kit

Cat. Num. AAA4502740

• Gene Names: SLC26A8; TAT1; SPGF3
• Reactivity: Human
• Synonyms: Testis Anion Transporter 1 (TAT1); Human Testis Anion Transporter 1 (TAT1) ELISA Kit; SLC26A8; Solute Carrier Family 26 Member 8; Anion exchange transporter; TAT1 rtu elisa kit

• Reactivity: Human
• Sequence Length: 970

• Assay Type: Sandwich
• Samples: Tissue Homogenates, Cell Lysates or Other Biological Fluids.
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.118ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TAT1 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TAT1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to TAT1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TAT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of TAT1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 301601599
• NCBI GeneID: 116369
• NCBI Accession #: NP_001180405.1
• NCBI GenBank Nucleotide #: NM_001193476.1
• UniProt Accession #: Q96RN1; Q5JVR5; Q812C7; Q8TC65; Q96MA0; Q96PK8
• Molecular Weight: 85,680 Da
• NCBI Official Full Name: testis anion transporter 1 isoform a
• NCBI Official Synonym Full Names: solute carrier family 26 member 8
• NCBI Official Symbol: SLC26A8
• NCBI Official Synonym Symbols: TAT1; SPGF3
• NCBI Protein Information: testis anion transporter 1
• UniProt Protein Name: Testis anion transporter 1
• Protein Family: Valine/tyrosine/tryptophan amino-acid permease
• UniProt Gene Name: SLC26A8

NCBI Description

This gene encodes a member of the SLC26 gene family of anion transporters. Family members are well conserved in gene structure and protein length yet have markedly different tissue expression patterns. The expression of this gene appears to be restricted to spermatocytes. Alternatively spliced transcript variants that encode different isoforms have been described. [provided by RefSeq, Jul 2010]

Uniprot Description

Acts as a DIDS-sensitive anion exchanger mediating chloride, sulfate and oxalate transport. May fulfill critical anion exchange functions in male germ line during meiosis and hence may play a role in spermatogenesis. May be involved in a new regulatory pathway linking sulfate transport to RhoGTPase signaling in male germ cells. A critical component of the sperm annulus that is essential for correct sperm tail differentiation and motility and hence male fertility. May form a moleculer complex involved in the regulation of chloride and bicarbonate ions fluxes during sperm capacitation.