Human Prostaglandin I Synthase ELISA Kit | PTGIS elisa kit

Human Prostaglandin I Synthase ELISA Kit

Cat. Num. AAA721938

• Gene Names: PTGIS; CYP8; PGIS; PTGI; CYP8A1
• Reactivity: Human
• Synonyms: Prostaglandin I Synthase; Human Prostaglandin I Synthase ELISA Kit; PTGIS elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 1.0-25ng/mL
• Sensitivity: 0.1ng/mL
• Highest Standards: 25ng/mL
• Concentration of Standards: Concentration of Standards
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PTGIS elisa kit

For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

INTENDED USE This PTGIS ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PTGIS. This ELISA kit for research use only!

PRINCIPLE OF THE ASSAYPTGIS ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PTGIS antibody and an PTGIS-HRP conjugate. The assay sample and buffer are incubated together with PTGIS-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PTGIS concentration since PTGIS from samples and PTGIS-HRP conjugate compete for the anti-PTGIS antibody binding site. Since the number of sites is limited, as more sites are occupied by PTGIS from the sample, fewer sites are left to bind PTGIS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PTGIS concentration in each sample is interpolated from this standard curve

NCBI and Uniprot Product Information

• NCBI GI #: 3308978
• NCBI GeneID: 5740
• Molecular Weight: 57,104 Da
• NCBI Official Full Name: prostacyclin synthase
• NCBI Official Synonym Full Names: prostaglandin I2 (prostacyclin) synthase
• NCBI Official Symbol: PTGIS
• NCBI Official Synonym Symbols: CYP8; PGIS; PTGI; CYP8A1
• NCBI Protein Information: prostacyclin synthase; prostaglandin I2 synthase; cytochrome P450, family 8, subfamily A, polypeptide 1
• UniProt Protein Name: Prostacyclin synthase
• Protein Family: Prostacyclin synthase
• UniProt Gene Name: PTGIS
• UniProt Synonym Gene Names: CYP8; CYP8A1
• UniProt Entry Name: PTGIS_HUMAN

NCBI Description

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. However, this protein is considered a member of the cytochrome P450 superfamily on the basis of sequence similarity rather than functional similarity. This endoplasmic reticulum membrane protein catalyzes the conversion of prostglandin H2 to prostacyclin (prostaglandin I2), a potent vasodilator and inhibitor of platelet aggregation. An imbalance of prostacyclin and its physiological antagonist thromboxane A2 contribute to the development of myocardial infarction, stroke, and atherosclerosis. [provided by RefSeq, Jul 2008]

Uniprot Description

PTGIS: Catalyzes the isomerization of prostaglandin H2 to prostacyclin (= prostaglandin I2). Belongs to the cytochrome P450 family.

Protein type: Lipid Metabolism - arachidonic acid; Isomerase; Endoplasmic reticulum; EC 5.3.99.4; Membrane protein, integral

Chromosomal Location of Human Ortholog: 20q13.13

Cellular Component: extracellular space; endoplasmic reticulum membrane; endoplasmic reticulum; integral to membrane; caveola; nucleus

Molecular Function: protein binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; iron ion binding; prostaglandin-I synthase activity; heme binding; monooxygenase activity

Biological Process: positive regulation of angiogenesis; vitamin metabolic process; nicotinamide metabolic process; inhibition of NF-kappaB transcription factor; NAD metabolic process; xenobiotic metabolic process; negative regulation of inflammatory response; negative regulation of nitric oxide biosynthetic process; icosanoid metabolic process; cyclooxygenase pathway; arachidonic acid metabolic process; prostaglandin biosynthetic process; water-soluble vitamin metabolic process

Disease: Hypertension, Essential