Rabbit anti-Human TBX21 Polyclonal Antibody | anti-TBX21 antibody

TBX21, NT (TBX21, TBET, TBLYM, T-box transcription factor TBX21, T-cell-specific T-box transcription factor T-bet, Transcription factor TBLYM) (Azide free) (HRP)

Cat. Num. AAA6354198

• Gene Names: TBX21; TBET; T-PET; T-bet; TBLYM
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TBX21; Polyclonal Antibody; NT (TBX21; TBET; TBLYM; T-box transcription factor TBX21; T-cell-specific T-box transcription factor T-bet; Transcription factor TBLYM) (Azide free) (HRP); Transcription factor TBLYM; anti-TBX21 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-TBX21 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: TBX21 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 21-50 amino acids from the N-terminal region of human TBX21.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunofluorescence (IF)

(Fluorescent image of A549 cell stained with TBX21 Antibody (N-term).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TBX21 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C).TBX21 immunoreactivity is localized to Nucleus significantly. )

Western Blot (WB)

(TBX21 Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the TBX21 antibody detected the TBX21 protein (arrow).)

Related Product Information for anti-TBX21 antibody

This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is the human ortholog of mouse Tbx21/Tbet gene. Studies in mouse show that Tbx21 protein is a Th1 cell-specific transcription factor that controls the expression of the hallmark Th1 cytokine, interferon-gamma (IFNG). Expression of the human ortholog also correlates with IFNG expression in Th1 and natural killer cells, suggesting a role for this gene in initiating Th1 lineage development from naive Th precursor cells.

Product Categories/Family for anti-TBX21 antibody

Antibodies; Transcription Factors

References

Mohr, E., et al. Proc. Natl. Acad. Sci. U.S.A. 107(40):17292-17297(2010). Zhu, Y., et al. J. Immunol. 185(6):3174-3183(2010). Qu, Y., et al. J. Immunol. 185(5):2895-2902(2010). Schuurhof, A., et al. Pediatr. Pulmonol. 45(6):608-613(2010). Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 7019549
• NCBI GeneID: 30009
• NCBI Accession #: NP_037483.1
• NCBI GenBank Nucleotide #: NM_013351.1
• UniProt Accession #: Q9UL17
• Molecular Weight: 58,328 Da
• NCBI Official Full Name: T-box transcription factor TBX21
• NCBI Official Synonym Full Names: T-box 21
• NCBI Official Symbol: TBX21
• NCBI Official Synonym Symbols: TBET; T-PET; T-bet; TBLYM
• NCBI Protein Information: T-box transcription factor TBX21; T-box protein 21; T-box expressed in T cells; transcription factor TBLYM; T-cell-specific T-box transcription factor T-bet
• UniProt Protein Name: T-box transcription factor TBX21
• Protein Family: T-box transcription factor
• UniProt Gene Name: TBX21
• UniProt Synonym Gene Names: TBET; TBLYM; T-box protein 21
• UniProt Entry Name: TBX21_HUMAN

NCBI Description

This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is the human ortholog of mouse Tbx21/Tbet gene. Studies in mouse show that Tbx21 protein is a Th1 cell-specific transcription factor that controls the expression of the hallmark Th1 cytokine, interferon-gamma (IFNG). Expression of the human ortholog also correlates with IFNG expression in Th1 and natural killer cells, suggesting a role for this gene in initiating Th1 lineage development from naive Th precursor cells. [provided by RefSeq, Jul 2008]

Uniprot Description

TBX21: Transcription factor that controls the expression of the TH1 cytokine, interferon-gamma. Initiates TH1 lineage development from naive TH precursor cells both by activating TH1 genetic programs and by repressing the opposing TH2 programs. T-cell specific.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 17q21.32

Cellular Component: cell soma; nucleus

Molecular Function: transcription factor activity

Biological Process: positive regulation of isotype switching to IgG isotypes; regulation of transcription, DNA-dependent; transcription, DNA-dependent; multicellular organismal development; response to virus; positive regulation of transcription, DNA-dependent; T cell differentiation

Disease: Asthma, Nasal Polyps, And Aspirin Intolerance