Human Proteasome 26S Subunit, Non ATPase 4 (PSMD4) RTU ELISA Kit | PSMD4 rtu elisa kit

Human Proteasome 26S Subunit, Non ATPase 4 (PSMD4) RTU ELISA Kit

Cat. Num. AAA4503858

• Gene Names: WNT2B; WNT13
• Reactivity: Human
• Purity: Affinity Purification
• Synonyms: Proteasome 26S Subunit; Non ATPase 4 (PSMD4); Human Proteasome 26S Subunit; Non ATPase 4 (PSMD4) RTU ELISA Kit; Multiubiquitin chain-binding protein; Antisecretory factor 1(AF;ASF); 26S proteasome regulatory subunit RPN10; 26S proteasome regulatory subunit S5A; PSMD4 rtu elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of PSMD4. No significant cross-reactivity or interference between PSMD4 and analogues was observed.
• Purity/Purification: Affinity Purification
• Sequence Length: 299

• Samples: Human Serum, Plasma, Tissue Homogenates Or Other Biological Fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 78-5000pg/ml
• Sensitivity: 29.8pg/mL.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PSMD4 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PSMD4 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PSMD4 rtu elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of PSMD4 in human serum, plasma, tissue homogenates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PSMD4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to PSMD4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PSMD4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of PSMD4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 630044901
• NCBI GeneID: 7482
• NCBI Accession #: NP_001278809.1
• NCBI GenBank Nucleotide #: NM_001291880.1
• UniProt Accession #: Q93097; O14903; Q5TEH9; Q5TEI2; Q9HDC1; Q9HDC2
• Molecular Weight: 41,830 Da
• NCBI Official Full Name: protein Wnt-2b isoform 3
• NCBI Official Synonym Full Names: Wnt family member 2B
• NCBI Official Symbol: WNT2B
• NCBI Official Synonym Symbols: WNT13
• NCBI Protein Information: protein Wnt-2b
• UniProt Protein Name: Protein Wnt-2b
• Protein Family: 26S proteasome non-ATPase regulatory
• UniProt Gene Name: WNT2B
• UniProt Synonym Gene Names: WNT13

NCBI Description

This gene encodes a member of the wingless-type MMTV integration site (WNT) family of highly conserved, secreted signaling factors. WNT family members function in a variety of developmental processes including regulation of cell growth and differentiation and are characterized by a WNT-core domain. This gene may play a role in human development as well as carcinogenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]

Uniprot Description

Ligand for members of the frizzled family of seven transmembrane receptors. Functions in the canonical Wnt/beta-catenin signaling pathway. Plays a redundant role in embryonic lung development.