Human Proteasome 26S Subunit, Non ATPase 4 (PSMD4) ELISA Kit | PSMD4 elisa kit

Human Proteasome 26S Subunit, Non ATPase 4 (PSMD4) ELISA Kit

Cat. Num. AAA4503839

• Gene Names: PSMD4; AF; ASF; S5A; AF-1; MCB1; Rpn10; pUB-R5
• Reactivity: Human
• Purity: Affinity Purification
• Synonyms: Proteasome 26S Subunit; Non ATPase 4 (PSMD4); Human Proteasome 26S Subunit; Non ATPase 4 (PSMD4) ELISA Kit; Multiubiquitin chain-binding protein; Antisecretory factor 1(AF/ASF); 26S proteasome regulatory subunit RPN10; 26S proteasome regulatory subunit S5A; PSMD4 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of PRSS3. No significant cross-reactivity or interference between PRSS3 and analogues was observed.
• Purity/Purification: Affinity Purification
• Sequence Length: 377

• Samples: Human Serum, Plasma, Tissue Homogenates Or Other Biological Fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 78.1-5000pg/mL
• Sensitivity: 26.9pg/mL.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PRSS3 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PRSS3 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: Store at 4 degree C or -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PSMD4 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of PRSS3 in human serum, plasma, tissue homogenates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PRSS3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to PRSS3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PRSS3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of PRSS3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 5292161
• NCBI GeneID: 5710
• NCBI Accession #: NP_002801.1
• NCBI GenBank Nucleotide #: NM_002810.2
• UniProt Accession #: P55036; Q5VWC5; Q9NS92; D3DV16
• Molecular Weight: 28,612 Da
• NCBI Official Full Name: 26S proteasome non-ATPase regulatory subunit 4 isoform 2
• NCBI Official Synonym Full Names: proteasome 26S subunit, non-ATPase 4
• NCBI Official Symbol: PSMD4
• NCBI Official Synonym Symbols: AF; ASF; S5A; AF-1; MCB1; Rpn10; pUB-R5
• NCBI Protein Information: 26S proteasome non-ATPase regulatory subunit 4
• UniProt Protein Name: 26S proteasome non-ATPase regulatory subunit 4
• Protein Family: 26S proteasome non-ATPase regulatory
• UniProt Gene Name: PSMD4
• UniProt Synonym Gene Names: MCB1; AF; ASF

NCBI Description

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the non-ATPase subunits of the 19S regulator lid. Pseudogenes have been identified on chromosomes 10 and 21. [provided by RefSeq, Jul 2008]

Uniprot Description

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. PSMD4 acts as an ubiquitin receptor subunit through ubiquitin-interacting motifs and selects ubiquitin-conjugates for destruction. Displays a preferred selectivity for longer polyubiquitin chains.