Human Procollagen C Proteinase Enhancer 1 (PCPE1) RTU ELISA Kit | PCPE1 rtu elisa kit

Human Procollagen C Proteinase Enhancer 1 (PCPE1) ELISA Kit

Cat. Num. AAA4501691

• Gene Names: PCOLCE; PCPE; PCPE1; PCPE-1
• Reactivity: Human
• Synonyms: Procollagen C Proteinase Enhancer 1 (PCPE1); Human Procollagen C Proteinase Enhancer 1 (PCPE1) ELISA Kit; PCOLCE; PCPE; Procollagen Type 1 COOH-Terminal Proteinase Enhancer; Procollagen C Endopeptidase Enhancer; Type I procollagen COOH-terminal proteinase enhancer; PCPE1 rtu elisa kit

• Reactivity: Human
• Sequence Length: 449

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates or Other Biological Fluids.
• Detection Range: 0.625-40ng/mL
• Sensitivity: 0.238ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PCPE1 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PCPE1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to PCPE1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PCPE1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PCPE1 in the samples is then determined by comparing the O.D. of the samples to the standard curve

NCBI and Uniprot Product Information

• NCBI GI #: 157653329
• NCBI GeneID: 5118
• NCBI Accession #: NP_002584.2
• NCBI GenBank Nucleotide #: NM_002593.3
• UniProt Accession #: Q15113; O14550; B2R9E1
• Molecular Weight: 47,972 Da
• NCBI Official Full Name: procollagen C-endopeptidase enhancer 1
• NCBI Official Synonym Full Names: procollagen C-endopeptidase enhancer
• NCBI Official Symbol: PCOLCE
• NCBI Official Synonym Symbols: PCPE; PCPE1; PCPE-1
• NCBI Protein Information: procollagen C-endopeptidase enhancer 1
• UniProt Protein Name: Procollagen C-endopeptidase enhancer 1
• UniProt Gene Name: PCOLCE
• UniProt Synonym Gene Names: PCPE1; PCPE-1; Procollagen C-proteinase enhancer 1

NCBI Description

Fibrillar collagen types I-III are synthesized as precursor molecules known as procollagens. These precursors contain amino- and carboxyl-terminal peptide extensions known as N- and C-propeptides, respectively, which are cleaved, upon secretion of procollagen from the cell, to yield the mature triple helical, highly structured fibrils. This gene encodes a glycoprotein which binds and drives the enzymatic cleavage of type I procollagen and heightens C-proteinase activity. [provided by RefSeq, Jul 2008]

Uniprot Description

Binds to the C-terminal propeptide of type I procollagen and enhances procollagen C-proteinase activity.