Typical Testing Data/Standard Curve (for reference only)
Human PCPE1 ELISA Kit | PCPE1 elisa kit
Human PCPE1 (Procollagen C Proteinase Enhancer 1) ELISA Kit
Gene Names
PCOLCE; PCPE; PCPE1; PCPE-1
Reactivity
Human
Synonyms
PCPE1; Human PCPE1 (Procollagen C Proteinase Enhancer 1) ELISA Kit; PCPE1 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human PCPE1. No significant cross-reactivity or interference between Human PCPE1 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human PCPE1 and all the analogues, therefore, cross reaction may still exist.
Sequence Length
449
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
0.625-40ng/mL
Sensitivity
Min: 0.375ng/mL; Max: 40ng/mL
Preparation and Storage
Store at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for PCPE1 elisa kit
Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PCPE1 in human serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PCPE1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human PCPE1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PCPE1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human PCPE1. You can calculate the concentration of Human PCPE1 in the samples by comparing the OD of the samples to the standard curve.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PCPE1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human PCPE1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PCPE1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human PCPE1. You can calculate the concentration of Human PCPE1 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
47,972 Da
NCBI Official Full Name
procollagen C-endopeptidase enhancer 1
NCBI Official Synonym Full Names
procollagen C-endopeptidase enhancer
NCBI Official Symbol
PCOLCE
NCBI Official Synonym Symbols
PCPE; PCPE1; PCPE-1
NCBI Protein Information
procollagen C-endopeptidase enhancer 1; procollagen C-proteinase enhancer 1; procollagen COOH-terminal proteinase enhancer 1; procollagen, type 1, COOH-terminal proteinase enhancer; type 1 procollagen C-proteinase enhancer protein; type I procollagen COOH-terminal proteinase enhancer
UniProt Protein Name
Procollagen C-endopeptidase enhancer 1
UniProt Gene Name
PCOLCE
UniProt Synonym Gene Names
PCPE1; PCPE-1; Procollagen C-proteinase enhancer 1
UniProt Entry Name
PCOC1_HUMAN
NCBI Description
Fibrillar collagen types I-III are synthesized as precursor molecules known as procollagens. These precursors contain amino- and carboxyl-terminal peptide extensions known as N- and C-propeptides, respectively, which are cleaved, upon secretion of procollagen from the cell, to yield the mature triple helical, highly structured fibrils. This gene encodes a glycoprotein which binds and drives the enzymatic cleavage of type I procollagen and heightens C-proteinase activity. [provided by RefSeq, Jul 2008]
Uniprot Description
PCOLCE: Binds to the C-terminal propeptide of type I procollagen and enhances procollagen C-proteinase activity.
Protein type: Secreted; Secreted, signal peptide; Extracellular matrix
Chromosomal Location of Human Ortholog: 7q22
Cellular Component: extracellular matrix; extracellular space
Molecular Function: heparin binding; collagen binding; protein binding; protease activator activity
Biological Process: multicellular organismal development; proteolysis
Research Articles on PCPE1
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Product Notes
The Human PCPE1 pcolce (Catalog #AAA2506338) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2506338 ELISA Kit recognizes Human PCPE1. It is sometimes possible for the material contained within the vial of "PCPE1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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