Human Plasminogen Activator, Urokinase Receptor (uPAR) RTU ELISA Kit | uPAR rtu elisa kit

Human Plasminogen Activator, Urokinase Receptor (uPAR) ELISA Kit

Cat. Num. AAA4503230

• Gene Names: PLAUR; CD87; UPAR; URKR; U-PAR
• Reactivity: Human
• Synonyms: Plasminogen Activator; Urokinase Receptor (uPAR); Human Plasminogen Activator; Urokinase Receptor (uPAR) ELISA Kit; CD87; PLAUR; PLAU-R; U-PAR; URKR; Monocyte Activation Antigen Mo3; uPAR rtu elisa kit

• Reactivity: Human
• Sequence Length: 281

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates or Other Biological Fluids.
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.117ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for uPAR rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to uPAR. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to uPAR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain uPAR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of uPAR in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 53829379
• NCBI GeneID: 5329
• NCBI Accession #: NP_001005376.1
• NCBI GenBank Nucleotide #: NM_001005376.2
• UniProt Accession #: Q03405; Q12876; Q15845; Q16887; Q6IB52; Q9BWT0; Q9NYC8; Q9UD69; Q9UEA6; Q9UM92; Q9UMV0; A8K409
• Molecular Weight: 32,016 Da
• NCBI Official Full Name: urokinase plasminogen activator surface receptor isoform 2
• NCBI Official Synonym Full Names: plasminogen activator, urokinase receptor
• NCBI Official Symbol: PLAUR
• NCBI Official Synonym Symbols: CD87; UPAR; URKR; U-PAR
• NCBI Protein Information: urokinase plasminogen activator surface receptor
• UniProt Protein Name: Urokinase plasminogen activator surface receptor
• UniProt Gene Name: PLAUR
• UniProt Synonym Gene Names: MO3; UPAR; U-PAR; uPAR

NCBI Description

This gene encodes the receptor for urokinase plasminogen activator and, given its role in localizing and promoting plasmin formation, likely influences many normal and pathological processes related to cell-surface plasminogen activation and localized degradation of the extracellular matrix. It binds both the proprotein and mature forms of urokinase plasminogen activator and permits the activation of the receptor-bound pro-enzyme by plasmin. The protein lacks transmembrane or cytoplasmic domains and may be anchored to the plasma membrane by a glycosyl-phosphatidylinositol (GPI) moiety following cleavage of the nascent polypeptide near its carboxy-terminus. However, a soluble protein is also produced in some cell types. Alternative splicing results in multiple transcript variants encoding different isoforms. The proprotein experiences several post-translational cleavage reactions that have not yet been fully defined. [provided by RefSeq, Jul 2008]

Uniprot Description

Acts as a receptor for urokinase plasminogen activator. Plays a role in localizing and promoting plasmin formation. Mediates the proteolysis-independent signal transduction activation effects of U-PA. It is subject to negative-feedback regulation by U-PA which cleaves it into an inactive form.