Human Large Multifunctional Peptidase 2 (LMP2) ELISA Kit | LMP2 elisa kit

Human Large Multifunctional Peptidase 2 (LMP2) ELISA Kit

Cat. Num. AAA2701664

• Gene Names: PSMB9; LMP2; PSMB6i; RING12; beta1i
• Reactivity: Human
• Synonyms: Large Multifunctional Peptidase 2 (LMP2); Human Large Multifunctional Peptidase 2 (LMP2) ELISA Kit; PSMB9; Proteasome(Prosome; Macropain)Subunit Beta Type 9; Macropain chain 7; Multicatalytic endopeptidase complex chain 7; Really interesting new gene 12 protein; LMP2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Large Multifunctional Peptidase 2 (LMP2).; No significant cross-reactivity or interference between Large Multifunctional Peptidase 2 (LMP2) and analogues was observed.
• Sequence Length: 219

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.057ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Large Multifunctional Peptidase 2 (LMP2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Large Multifunctional Peptidase 2 (LMP2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LMP2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Large Multifunctional Peptidase 2 (LMP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Large Multifunctional Peptidase 2 (LMP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Large Multifunctional Peptidase 2 (LMP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Large Multifunctional Peptidase 2 (LMP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 34635
• NCBI GeneID: 5698
• UniProt Accession #: P28065; Q16523; Q5JNW4; B0V0T1
• Molecular Weight: 22,328 Da
• NCBI Official Full Name: LMP2
• NCBI Official Synonym Full Names: proteasome subunit beta 9
• NCBI Official Symbol: PSMB9
• NCBI Official Synonym Symbols: LMP2; PSMB6i; RING12; beta1i
• NCBI Protein Information: proteasome subunit beta type-9
• UniProt Protein Name: Proteasome subunit beta type-9
• Protein Family: Latent membrane protein
• UniProt Gene Name: PSMB9
• UniProt Synonym Gene Names: LMP2; PSMB6i; RING12

NCBI Description

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 1 (proteasome beta 6 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. [provided by RefSeq, Mar 2010]

Uniprot Description

PSMB9: The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB6 by PSMB9 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues. Belongs to the peptidase T1B family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.25.1; Nuclear receptor co-regulator; Protease; Proteasome complex

Chromosomal Location of Human Ortholog: 6p21.32

Cellular Component: cytosol; nucleoplasm; proteasome complex; proteasome core complex

Molecular Function: protein binding; threonine endopeptidase activity

Biological Process: anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; MAPK cascade; negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; post-translational protein modification; proteasomal ubiquitin-dependent protein catabolic process; protein deubiquitination; protein polyubiquitination; regulation of cellular amino acid metabolic process; regulation of mRNA stability; SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; stimulatory C-type lectin receptor signaling pathway; T cell receptor signaling pathway; transmembrane transport; tumor necrosis factor-mediated signaling pathway; viral process; Wnt receptor signaling pathway, planar cell polarity pathway